Bpsbioscience/人肿瘤坏死因子α/90244-B/50µg
商品编号：
90244-B
品牌：
bpsbioscience
市场价：
¥4100.00
美元价：
2460.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:17kDa
Genbank#:P01375
a.a:77–233
Background:
TumorNecrosisFactorissecretedbymacrophages,monocytes,neutrophils,T-cells,andNK-cellsfollowingtheirstimulationbybacteriallipopolysaccharides.CellsexpressingCD4secreteTNF-alphawhileCD8(+)cellssecretelittleornoTNF-alpha.Stimulatedperipheralneutrophilicgranulocytesbutalsounstimulatedcellsandalsoanumberoftransformedcelllines,astrocytes,microglialcells,smoothmusclecells,andfibroblastsalsosecreteTNF.Humanmilkalsocontainsthisfactor.ThesynthesisofTNF-alphaisinducedbymanydifferentstimuliincludinginterferons,IL-2,GM-CSF,SP,Bradykinin,Immunecomplexes,inhibitorsofcyclooxygenaseandPAF(plateletactivatingfactor).HumanTNF-alphaisanon-glycosylatedproteinof17kDaandalengthof157aminoacids.MurineTNF-alphaisN-glycosylated.HomologywithTNF-betaisapproximately30%.TNF-alphaformsdimersandtrimers.
Description:
RecombinantTNF-alphaisadisulfide-linkedmonomerprotein,consistingof158aminoacidsandmigratesasanapproximately17kDaproteinunderreducingconditions.OptimizedDNAsequenceencodingHumanTumorNecrosisFactor-alphachainwasexpressedinE.coli.
UniProt
P01375
Synonym(s):
TNF-alpha,TNFa,TumorNecrosisFactor-alpha,Cachectin
Purity:
≥95%bySDS-PAGEandHPLC
EndotoxinLevel:
<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.
BIOLOGicalActivity:
TheED50wasdeterminedcytolysisofmurineL929cellsinthepresenceofActinomycinDis≤0.05ng/ml,correspondingtoaspecificactivityof>1.9x107units/mg.
Formulation:
Lyophilizedfroma0.2µmfilteredconcentrated(1mg/ml)solutioninPBS,pH7.2.
Format:
lyophilizedprotein
Reconstitution:
Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.
Storage/StABIlity:
Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.
Reference(s):
1.Vermel"AE.KlinMed(Mosk).2005,83(11):13-7.
2.TraceyKJ,CeramiA.AnnuRevMed.1994,45:491-503
3.SedgwickJD,etal.ImmunolToday.2000Mar,21(3):110-3.
2.TraceyKJ,CeramiA.AnnuRevMed.1994,45:491-503
3.SedgwickJD,etal.ImmunolToday.2000Mar,21(3):110-3.
Warning(s):
Avoidfreeze/thawcycles
AminoAcidSequence:
VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
ScientificCategory:
Cytokine/GrowthFactors
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
