Bpsbioscience/TMEM16A-HEK293重组细胞系/90230/2小瓶-免疫在线蚂蚁淘旗下平台

当前位置：首页 > 产品中心 > Routine_cell_strain > Bpsbioscience/TMEM16A-HEK293重组细胞系/90230/2小瓶

商品详细Bpsbioscience/TMEM16A-HEK293重组细胞系/90230/2小瓶

Bpsbioscience/TMEM16A-HEK293重组细胞系/90230/2小瓶

商品编号： 90230

品牌： bpsbioscience

市场价： ¥173000.00

美元价： 103800.00

产地：美国(厂家直采)

公司：

产品分类：常规细胞株

公司分类： Routine_cell_strain

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

立即询价

商品介绍

Species:Human

Genbank#:NM_018043

Background:

Calcium-activatedchloridechannels(CaCCs)aremajorregulatorsinnumerousphysiologicalprocessesincludingsensorytransduction,epithelialsecrection,cardiacandneutonalexcitation,andsmoothmusclecontraction.TMEM16A(ANO1),amemberofafamilyofputativeplasmamembraneproteins,isidentifiedasaCaCCthatisactivatedbyintracellularCa²⁺andCa²⁺-mobilizingstimuli.Ithaseightputativetransmembranesegmentswithoutdomainsevidentlyinvolvedincalciumregulation.TherelativepermeABIlityofTMEM16AtomonovalentanionsisNO₃^—>I^—>Br^—>Cl^—>F^—.

Description:

Stable,recombinantHEK293celllineexpressinghumanTMEM16A(transmembraneprotein16A,alsocalledanoctamin1,calcium-activatedchloridechannel(ANO1),accessionnumberNM_018043).

UniProt

Q5XXA6

Synonym(s):

TMEM16A,transmembraneprotein16A,anoctamin1,calcium-activatedchloridechannel,ANO1

SuppliedAs:

Eachvialcontains~1X10⁶cellsin1mlof10%DMSO.

BIOLOGicalActivity:

N-terminalFLAGtaggedhumanTMEM16AchannelhasbeenstablyexpressedinHEK293celllineanditsexpressionwasconfirmedbyWesternblotting.TheCaCCactivityofTMEM16Awascharacterizedbyanassaybasedonhalide-sensitiveyellowfluorescentprotein(YFP)mutantwhosefluorescenceisquenchedbyincreasinghalideconcentration.WhenTMEM16A-expressedHEK293cellswerestimulatedwithionomycintoraisetheintracellularlevelofCa²⁺,TMEM16AproducedI^–influxinHEK293thattriggeredtherapiddecreaseoffluorescenceoftransfectedYFPmutant.Theionomycin-inducedI^–influxthroughTMEM16Awasblockedbyniflumicacid,aCaCCchannelblocker.

HostCellline:

HEK293cells

MycoplasmaTesting:

ThecelllinehasbeenscreenedusingthePCR-basedVenorGeMMycoplasmaDetectionkit(SigmaAldrich)toconfirmtheabsenceofMycoplasmaspecies.

Instructionsforuse:

Seedatasheetfordetailedculturingandassayprotocol.

Storage/Stability:

Storeinliquidnitrogenimmediatelyuponreceipt.

Application(s):

•DrugcompoundscreeningforactivatorsorinhibitorsofTMEM16A
•FunctionalassaysforTMEM16A
•Efficientantigenformouseimmunization

Reference(s):

1.VerkmanA.S.andGaliettaL.J.V.(2009)Cholidechannelsasdrugtargets.NatureReviews8:153-171.
2.CaputoA.etal.(2008).Science322:590-594.
3.YangY.D.etal.(2008).Nature455:1210-1215.

Notes:

Contactusforpricing.

LicenseDisclosure:Purchaseofthiscelllinegrantsyouwitha10-yearlicensetousethiscelllineinyourimmediatelaboratory,forresearchuseonly.Thislicensedoesnotpermityoutoshare,distribute,sell,sublicense,orotherwisemakethecelllineavailableforusetootherlaboratories,departments,researchinstitutions,hospitals,universities,orbiotechcompanies.Thelicensedoesnotpermituseofthiscelllineinhumansorfortherapeuticordruguse.Thelicensedoesnotpermitmodificationofthecelllineinanyway.InappropriateuseordistributionofthiscelllinewillresultinrevocationofthelicenseandresultinanimmediateceaseofsalesanddistributionofBPSproductstoyourlaboratory.BPSdoesnotwarrantthesuitabilityofthecelllineforanyparticularuse,anddoesnotacceptanyliabilityinconnectionwiththehandlingoruseofthecellline.Modificationsofthiscellline,transfertoanotherfacility,orcommercialuseofthecellsmayrequireaseparatelicenseandadditionalfees;contactsales@bpSBIoscience.comfordetails.PublicationsusingthiscelllineshouldreferenceBPSBioscience,Inc.,SanDiego.

Warning(s):

Avoidfreeze/thawcycles

ScientificCategory:

IonChannel

ProductType:

CellLine

Datashownislot-specific.Contactusforspecificinformationonotherlots.

品牌介绍

bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。

公司介绍

联络我们

服务热线：4000-520-616

（限工作日9:00-18:00）

QQ ：1570468124

手机：18915418616

官网：http://www.bpsbioscience.com