Bpsbioscience/TIGIT:CD112 Homogeneous Assay Kit/72030/384 reactions
商品编号：
72030
品牌：
bpsbioscience
市场价：
¥6700.00
美元价：
4020.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Background:
HumanT-cellimmunoreceptorwithIgandITIMdomains(TIGIT)isareceptorthatisexpressedonthesurfaceofhumanTcellsandNKcellsthatbindstoCD155andCD112onthesurfaceofdendriticcells.BindingofTIGITwithCD155orCD112resultsininhibitionofTcellandNKcellactivation.Antibodiesandotheragentsthatinhibitthissignalingpathwayhavebeenshowntoincreasetheimmuneresponse,especiallyinthecaseofcertaincancers.
Description:
TheTIGIT:CD112HomogeneousAssayKitisdesignedtomeasuretheinhibitionofhumanT-cellimmunoreceptorwithIgandITIMdomains(TIGIT)bindingtoCD112(PVRL2/Nectin-2).TheTIGIT:CD112HomogeneousAssayKitcomesinaconvenientAlphaLISA®formatwithpurifiedbiotinylatedTIGIT,His-taggedCD112,andassaybuffertoperformatotalof384reactions.Withthiskit,onlythreesimplestepsonamicrotiterplatearerequired.First,asamplecontainingTIGITandaninhibitorofchoiceisincubatedwiththeCD112for60minutes.Next,acceptorbeadsareadded,thendonorbeads,followedbyreADIngtheAlpha-counts.
Synonym(s):
T-cellimmunoreceptorwithIgandITIMdomains,V-setandimmunoglobulindomain-containingprotein9(VSIG9),andV-setandtransmembranedomaincontainingprotein3(VSTM3),PVRL2
Contraindications:
OnlylimitedamountsofDMSOcanbeincluded,asithasbeenshowntodisruptTIGIT:CD112interaction.AvoidgreenandbluedyesthatabsorblightintheAlphaScreensignalemissionrange(520-620nm),suchasTrypanBlue.Avoidtheuseofthepotentsingletoxygenquencherssuchassodiumazide(NaN3)ormetalions(Fe2+,Fe3+,Cu2+,Zn2+andNi2+).Thepresenceof>1%RPMI1640culturemediumleadstoasignalreductionduetothepresenceofexcessbiotinandironinthismedium.MEM,whichlacksthesecomponents,doesnotaffectAlphaScreenassays.
Format:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
Atleastoneyearfromdateofreceiptwhenstoredasdirected.
Application(s):
UsefulforscreeningforinhibitorsofTIGITbindingtoCD112
Reference(s):
1.Yu,X.,etal.,Nat.Immunol.2009;10(1):48-57.
2.Stanietsky,N.,etal.,Proc.Natl.Acad.Sci.2009;106(42):17858-17863.
Notes:
MATERIALSORINSTRUMENTSREQUIREDBUTNOTSUPPLIED:
AlphaLISANiChelateAcceptorbeads,5mg/ml(Perkinelmer#AL108C)
AlphaScreenStreptavidin-conjugatedDonorbeads,5mg/ml(PerkinElmer#6760002S)
Optiplate-384(PerkinElmer#6007290)
AlphaScreenmicroplatereader
Adjustablemicropipettorandsteriletips
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Immunotherapy/CellSurfaceReceptor
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
