Bpsbioscience/SMYD3均相分析试剂盒/52063/384反应
商品编号:
52063
品牌:
bpsbioscience
市场价:
¥21400.00
美元价:
12840.00
产品分类:
其它检测试剂盒
公司分类:
Other_kits
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Description:
TheSMYD3HomogeneousAssayKitisdesignedtomeasureSMYD3activityforscreeningandprofilingapplications.SMYD3isahistonemethyltransferasethatpromotesdiandtrimethylationoflysine4ofhistoneH3.ItalsoexhibitsmethylationactivitytowardalysineresidueofMAPK/ERKKinaseKinase2(MAP3K2).ThekeytotheSMYD3HomogeneousAssayKitisahighlyspecificantibodythatrecognizesmethylatedsubstrate.Withthiskit,onlythreesimplestepsonamicrotiterplatearerequiredformethyltransferasedetection.First,asamplecontainingSMYD3enzymeisincubatedwiththesubstrateforthreehours.Next,acceptorbeadsandprimaryantibodyareadded,thendonorbeads,followedbyreADIngtheAlpha-counts.
Synonym(s):
KMT3E
SuppliedAs:
TheSMYD3HomogeneousAssayKitcomesinaconvenientAlphaLISA®format,withGST-taggedSMYD3substrate,primaryantibody,methylationassaybuffer,andpurifiedSMYD3for384enzymereactions.
Contraindications:
GreenandbluedyesthatabsorblightintheAlphaScreen®signalemissionrange(520-620nm),suchasTrypanBlue.Avoidtheuseofthepotentsingletoxygenquencherssuchassodiumazide(NaN3)ormetalions(Fe2+,Fe3+,Cu2+,Zn2+andNi2+).Thepresenceof>1%RPMI1640culturemediumleadstoasignalreductionduetothepresenceofexcessbiotinandironinthismedium.MEM,whichlacksthesecomponents,doesnotaffectAlphaScreen®assays.
Format:
COMPONENTS:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
12monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymekineticsandHTSapplications.
Reference(s):
1.Dillon,S.C.,etal.2005.GenomeBIOLOGy6:227.
2.Silva,F.P.,etal.2008.Oncogene27:2686.
2.Silva,F.P.,etal.2008.Oncogene27:2686.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Methyltransferase
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。