Bpsbioscience/MLL1/WDR5/Ash2L/RbBP5/DPY30 (MLL1/WARD Complex), FLAG, His-tag/51021/50 µg
商品编号：
51021
品牌：
bpsbioscience
市场价：
¥13400.00
美元价：
8040.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:195kDa(complex)
Genbank#:NM_005933(MLL1),NM_017588(WDR5),NM_001105214(Ash2L),NM_005057(RbBP5),NM_032574(DPY30)
Tag(s):N-terminalFLAG-tag(WDR5),N-terminalHis-tag(others)
a.a:MLL3745–end,WDR53745–end,othercomponents2–end
Background:
MLLisahistonemethyltransferasethatplaysanessentialroleinearlydevelopmentandhematopoiesis.PartofamultiproteincomplexthatmediatesbothmethylationofLys4ofhistoneH3(H3K4me)complexandacetylationofLys16ofhistoneH4(H4K16ac).Hasweakmethyltransferaseactivitybyitself,andrequiresothercomponentoftheMLL1/MLLcomplextoobtainfullmethyltransferaseactivity.Multiplechromosomaltranslocationsinvolvingthisgenearethecauseofcertainacutelymphoidleukemiasandacutemyeloidleukemias.
Description:
MLL1/WARDcomplexofhumanMLL1(GenBankAccessionNo.NM_005933,)a.a.3745-endwithN-terminalHis-tagandMW=27kDa,humanWDR5(GenBankAccessionNo.NM_017588),a.a.22-endwithN-terminalFLAG-tagandMW=35kDa,humanAsh2L(GenBankAccessionNo.NM_001105214),a.a.2-endwithN-terminalHis-tagandMW=61kDa,humanRbBP5(GenBankAccessionNo.NM_005057),a.a.2-endwithN-terminalHis-tagandMW=60kDa,andhumanDPY30(GenBankAccessionNo.NM_032574)a.a.2-endwithN-terminalHis-tagandMW=12kDaindividuallyexpressedinanE.coliexpressionsystem.
UniProt
Q03164(MLL1),P61964(WDR5),Q9UBL3(Ash2L),Q15291(RbBP5),Q9C005(DPY30)
Synonym(s):
MLL,Myeloid/LymphoidOrMixed-LineageLeukemia,HRX,ALL-1,CXXC7,WDSTS,KMT2A,TRX1,MLL1;
AssayConditions:
50µlreactionmix(50mMTris,pH8.8,5mMMgCl2,4MDTT,20µMS-adenosylmethionine,and5-50ngMLLcomplex)addtothewellscoatedwiththesubstrate.Incubateatroomtemperaturefor1hr.AddantibodyagainstmethylatedK4residueofhistoneH3,incubate1hr.Then,incubate30minuteswiththesecondaryHRP-labeledantibody.Finally,addHRPchemiluminescentsubstrateandreadluminescence.
Formulation:
45mMTris-HCl,pH8.0,124mMNaCl,2.4mMKCl,225mMimidazole,1mMTCEP,1µMZnCl2,and10%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Patel,A.,etal.,J.Biol.Chem.2009;284(36):24242-24256.
2.Mohan,M.,etal.,Nat.Rev.Cancer2010;10(10):721-728.
2.Mohan,M.,etal.,Nat.Rev.Cancer2010;10(10):721-728.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Methyltransferase
ProductType:
RecombinantProteinComplex
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
