Bpsbioscience/HIPK1，GST标签/40179/10µg
商品编号：
40179
品牌：
bpsbioscience
市场价：
¥7800.00
美元价：
4680.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:71kDa
Genbank#:NM_152696
Tag(s):N-terminalGST-tag
a.a:156–555
Background:
HIPK1orhomeodomain-interactingproteinkinase1isaser/thrproteinkinaseandamemberoftheHIPKfamily.HIPK1isanuclearkinasethatphosphorylateshomeodomaintranscriptionfactors.HIPK1phosphorylatesDAXXandthisleadstoitsrelocalizationandsubsequentdecreaseintranscriptionalrepressionactivity(1).HIPK1alsointeractswithp53andphosphorylatesitonserineresidues.HIPK1expressioniselevatedinbreastcancercelllinesandembryonicfibroblastsfromHIPK1-nullmiceshowmoresusceptibilitytoapoptosisinducedbyDNAdamage(2).
Description:
HumanHIPK1(homeodomain-interactingproteinkinase1),alsoknownasMyakandNbak2(GenBankAccessionNo.NM_152696),a.a.156-555withN-terminalGSTtag,MW=71kDa,expressedinSf9insectcellsviaabaculovirusexpressionsystem.
Synonym(s):
Myak,Nbak2,KIAA0630,MGC26642,MGC33446,MGC33548
Purity:
≥90%
SpecificActivity:
79pmol/min/µg
AssayConditions:
5mMMOPS,2.5mMβ-glycerophosphate,5mMMgCl2,1mMEGTA,0.4mMEDTA,0.05mMDTT,2mMATP.IncubateHIPK1with0.25mg/mlMyelinbasicProtein(substrate)and50?M[33P]-ATPat30°Cfor15minutes,thenspotreactiononphosphocellulosepaper,fixin1%phosphoricacid,andassaywithascintillationcounter.
Formulation:
50mMTris-HCl,pH7.5,150mMNaCl,0.25mMDTT,0.1mMEGTA,0.1mMEDTA,0.1mMPMSF,25%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Ecsedy,JA.etal:Homeodomain-interactingproteinkinase1modulatesDaxxlocalization,phosphorylation,andtranscriptionalactivity.MolCellBiol.2003Feb;23(3):950-60.
2.Kondo,S.etal:Characterizationofcellsandgene-targetedmicedeficientforthep53-bindingkinasehomeodomain-interactingproteinkinase1(HIPK1).ProcNatlAcadSciUSA.2003Apr29;100(9):5431-6.
2.Kondo,S.etal:Characterizationofcellsandgene-targetedmicedeficientforthep53-bindingkinasehomeodomain-interactingproteinkinase1(HIPK1).ProcNatlAcadSciUSA.2003Apr29;100(9):5431-6.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/Serine-Threonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
