Bpsbioscience/GSK3β/40007/10微克
商品编号：
40007
品牌：
bpsbioscience
市场价：
¥7300.00
美元价：
4380.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 73 kDa
Genbank #: NM_002093
Tag(s): N-terminal GST-tag
a.a: full length
Description:
Human GSK3β GenBank Accession No. NM_002093), full length, with N-terminal GST-tag, MW=73 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
P49841
Synonym(s):
GSK3b, glycogen synthase kinase 3 beta, GSK3beta
Specific Activity:
391 pmol/min/µg
Assay Conditions:
Enzyme reactions include 50 μL
volumes of 1x Kinase buffer (40 mM Tris
pH 7.4, 20 mM MgCl2, 0.1 mg/mL BSA)
along with 20 μM ATP, 0.2 mg/mL GSKtide
substrate, 3mM DTT, and GSTGSK3
. After 45 min incubation at
30°C, 50μL of the Kinase-Glo reagent
solution was added and luminescence
from remaining ATP was measured.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Sutherland C. et al. Biochem J. 1993 Nov 15;296 (Pt 1):15-9.
2. Sperber BR et al. Neurosci Lett. 1995 Sep 8;197(2):149-53.
Application Reference(s):
1. Glycogen synthase kinase-3β inhibition in the medial prefrontal cortex mediates paradoxical amphetamine action in a mouse model of ADHD
(2015)
2. Induction of Gsk3β-β-TrCP interaction is required for late phase stabilization of β-catenin in canonical Wnt signaling (2014)
3. Leucine-rich repeat kinase 2 regulates tau phosphorylation through direct activation of glycogen synthase kinase-3β
(2014)
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Kinase/Serine-Threonine
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
