Bpsbioscience/ERN1 (IRE1), GST-tag/40306/10 µg
商品编号：
40306
品牌：
bpsbioscience
市场价：
¥7800.00
美元价：
4680.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Baculovirus-infectedSf9insect
MW:87kDa
Genbank#:No.NM_001433
Tag(s):N-terminalGST-tag
a.a:468-977(end)
Description:
Humanendoplasmicreticulumto-nucleussignaling1(ERN1),alsoknownasinositol-requiringprotein1(IRE1),GenBankAccessionNo.NM_001433,a.a.468-977(end)MW=87kDa,expressedinabaculovirus-infectedSf9insectcellexpressionsystem.
UniProt
O75460
Synonym(s):
inositol-requiringprotein1(IRE1),IRE
SpecificActivity:
55pmol/min/μg
AssayConditions:
10μlofvaryingamountsofERN1wasmixedwith5μlof1mg/mlmyelinbasicproteinsubstrateand5μlofH2O.Reactionwasinitiatedbyadding5μlof250μM[33P]-ATPandincubatingfor15minat30°Cfollowedbyspottingthereactiononphosphocellulosepaper,fixingin1%phosphoricacid,andassayingwithascintillationcounter.
Formulation:
50mMTris-HCl,pH7.5,150mMNaCl,10mMglutatione,0.1mMEDTA,0.25mMDTT,0.1mMPMSF,25%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C
Application(s):
Usefulforstudyingenzymekineticsandscreeningsmallmoleculeinhibitors.
Reference(s):
1.Niwa,M.,etal.,Cell.1999;99:691-702.
2.Iwawaki,T.etal.,Proc.Nat.Acad.Sci.2009;106:16657-16662.
Warning(s):
Avoidfreeze/thawcycles.Storingdilutedproteinisnotrecommended,ifnecessary,usecarrierprotein(BSA0.1–0.5%).
ScientificCategory:
Kinase/SerineThreonine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
