Bpsbioscience/EPHA4, GST-tag/40193/10 µg
商品编号：
40193
品牌：
bpsbioscience
市场价：
¥7100.00
美元价：
4260.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:58kDa
Genbank#:NM_004438
Tag(s):N-terminalGST-tag
a.a:610–887
Background:
EPHA4alsoknownasEPHreceptorA4,belongstotheephrinreceptorsubfamilyofprotein-tyrosinekinaseswhichhavebeenimplicatedinmediatingdevelopmentalevents,particularlyinthenervoussystem(1).TheEPHA4ligandephrin-A3islocalizedtotheastrocyticprocessesthatenvelopthespine.ActivationofEPHA4byephrin-A3inducesspinalretractionandreducesspinedensityandinhibitstheinteractiondistortedspineshapeandorganization.EPHA4-nullmicepossessdefectsinthecorticospinaltractandanteriorcommissureindicatingamodelinwhichanephrinligandontheaxonssensesEPHA4onspinalcordcellssurroundingthecorticospinaltract(2).
Description:
HumanEPHA4(EphrinReceptorA4),alsoknownasTYRO1,SEK,andHEK8,GenBankAccessionNo.NM_004438,a.a.610-887,withN-terminalGST-tag,MW~58kDa,expressedinSf9insectcellsviaabaculovirusexpressionsystem.
Synonym(s):
SEK,HEK8,TYRO1
Purity:
≥90%
SpecificActivity:
61pmol/min/µg
AssayConditions:
AssaywasdoneinKinasebuffercontaining0.2mMDTTusingPoly-(Glu4:Tyr)-biotinsubstrate(0.2mg/ml)and20µMATP.Reactionwasdoneat30°Cfor40min.AmountofATPtransferredwascalculatedusingKinase-Gloreagent(Promega).
Formulation:
50mMTris-HCl,pH7.5,150mMNaCl,10mMglutathione,0.1mMEDTA,0.25mMDTT,0.1mMPMSF,25%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Flanagan,J.G.etal:TheephrinsandEphreceptorsinneuraldevelopment.".Annu.Rev.Neurosci.1998;21:309–45.
2.Dottori,M.etal:EphA4(Sek1)receptortyrosinekinaseisrequiredforthedevelopmentofthecorticospinaltract.Proc.Nat.Acad.Sci.95:13248-13253,1998.
2.Dottori,M.etal:EphA4(Sek1)receptortyrosinekinaseisrequiredforthedevelopmentofthecorticospinaltract.Proc.Nat.Acad.Sci.95:13248-13253,1998.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Kinase/ReceptorTyrosine
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
