CD39contributestoimmuneregulationthroughthehydrolysisofATPtoAMPintheratelimitingstepofextracellularadenosineproduction.CD39isexpressedonthesurfaceofmultiplecellsoftheimmunesystemincludingT-cells,Naturalkillercells,B-cells,dendriticcellsandmacrophages.Inaddition,highlevelsofCD39expressionhavebeenassociatedwiththetumormicroenvironment(TME).ResearchaimedatreducingimmunosuppressionbyCD39activityhasresultedinthestudyofasmallmoleculeinhibitor,POM1,andmultipleanti-CD39antibodiesselectedforblockingenzymaticactivity ordecreasingCD39expression.
HumanCD73protein,alsoknownas5"-nucleotidaseandEcto-5"-nucleotidase,GenBankAccessionNo.NM_002526,a.a.27-547withC-terminalHis-tag,expressedinaHEK293cellexpressionsystem.MW=58.6kDa.ThisproteinrunsatahigherMWbySDS-PAGEduetoglycosylation.
2.Thompson,L.F.,etal.,J.Exp.Med.2004;200(11):1395-1405.
HiP™indicatesahighpurityproteinwith≥90%purityasmeasuredbygelfiltration.