Bpsbioscience/Human MCP-2(CCL8)/90213-B/25 µg
商品编号：
90213-B
品牌：
bpsbioscience
市场价：
¥4100.00
美元价：
2460.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: E. coli
MW: 9 kDa
Genbank #: P80075
a.a: 29–109
Background:
Monocyte chemotactic protein MCP-2 is a C-C chemokine.It shares over 60% amino acid identity with MCP-1 and MCP-3 and has about 30% identity with other C-C chemokines MIP-1alpha, RANTES, and MIP-1beta. MCP-2 is chemotactic for and activates a wide variety of inflammatory cells, and its spectrum of action on leukocytes is similar to that of MCP-3, including monocytes, T lymphocytes, NK cells, basophils, mast cells, and eosinophils, but differs from MCP-1, which is not active on eosinophils. MCP-2 has been proposed to interact with multiple C-C chemokine receptors, including those used by MCP- 1 and MCP-3. However, since leukocytes express a multiplicity of chemokine receptors with promiscuous binding and functional properties, it is difficult to identify the receptors used by a given ligand on these cells.
Description:
Recombinant MCP-2 is a disulfide-linked monomeric protein consisting of 77 amino acid residues and migrates as an approximately 9 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding human MCP-2 (CCL8) mature chain was expressed in E. coli.
UniProt
P80075
Synonym(s):
MCP-2, CCL8, Chemokine (C-C Motif) Ligand 8, Monocyte Chemoattractant Protein 2, HC14, SCYA10, MCP2
Purity:
≥98% by SDS-PAGE and HPLC
Endotoxin Level:
<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Biological Activity:
The ED50 was determined by the dose-dependent chemo-attraction of THP1 leukemic cells and was found to be ≤ 50 ng/ml, corresponding to a specific activity of >5x 104 units/mg.
Formulation:
Lyophilized from a 0.2 µm filtered 20 mM Phosphate buffer, 100 mM NaCl, pH 7.5.
Format:
lyophilized protein
Reconstitution:
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability:
The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Reference(s):
1. J. Immunol., Jan 2009, 182: 522 - 529.
2. Eur. Respir. J., Dec 2008, 32: 1607 - 1615.
3. Journal of Renin-Angiotensin-Aldosterone System, Mar 2007, 8: 45 - 50.
2. Eur. Respir. J., Dec 2008, 32: 1607 - 1615.
3. Journal of Renin-Angiotensin-Aldosterone System, Mar 2007, 8: 45 - 50.
Warning(s):
Avoid freeze/thaw cycles
Amino Acid Sequence:
SIPITCCFNVINRKIPIQRLESYTRITNIQCPKEAVIFKTKRGKEVCADPKERWVRDSMKHLDQIFQNLKP
Scientific Category:
Cytokine/Growth Factors
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
