Bpsbioscience/Human Interleukin-7/90198-B/10 µg
商品编号：
90198-B
品牌：
bpsbioscience
市场价：
¥4100.00
美元价：
2460.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: E. coli
MW: 17 kDa
Genbank #: P13232
a.a: 26–177
Background:
IL-7 is secreted constitutively into the conditioned medium of adherent bone marrow stromal cells and thymic cells. Mouse and human keratinocytes have been shown also to express and secrete IL-7. Human (152 amino acids) and murine IL-7 (129 amino acids) show 60% sequence homology at the protein level. The human IL-7 receptor, designated CD127, is an integral strongly glycosylated membrane protein of 76 kDa expressed on activated T-cells. IL-7 receptors are expressed on pre-B-cells and their progenitors and bone marrow macrophages, but they are not expressed on mature B-cells. Functional IL-7 receptors are also found on the cell surface of multiphenotypic, biphenotypic, and immature lymphoid progenitors of B-cells with the gene arrangement of the heavy immunoglobulin chain such as those observed in the germ line.
Description:
Recombinant human IL-7 is a disulfide-linked monomeric protein consisting of 153 amino acid residues, and migrates as an approximately 17 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE. Optimized DNA sequence encoding Human Interleukin-7 mature chain was expressed in E. coli.
UniProt
P13232
Synonym(s):
IL-7, Interleukin 7, IL7
Purity:
≥97% by SDS-PAGE and HPLC
Endotoxin Level:
<0.1 ng/µg (1 EU/µg), using the LAL gel clot method.
Biological Activity:
The ED50 determined by dose-dependent stimulation of the proliferation of murine 2E8 cells is ≤ 0.5 ng/ml, corresponding to a specific activity of ≥ 2 x 106 units/mg.
Formulation:
Lyophilized from a 0.2 µm filtered PBS solution, pH 4.5
Format:
lyophilized protein
Reconstitution:
Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage / Stability:
The lyophilized protein is stable for at least 2 years from date of receipt when stored at -20°C. Upon reconstitution, store in working aliquots at +4°C for up to one month, or at -20°C for up to six months, in the presence of a carrier protein. Avoid repeated freeze/thaw cycles.
Reference(s):
1. J. Exp. Med., Sep 2009, 206: 2111 - 2119.
2. J. Immunol., Sep 2009, 183: 3130 - 3138.
3. Blood, Aug 2009, 114: 1768 - 1775.
2. J. Immunol., Sep 2009, 183: 3130 - 3138.
3. Blood, Aug 2009, 114: 1768 - 1775.
Warning(s):
Avoid freeze/thaw cycles
Amino Acid Sequence:
DCDIEGKDGKQYESVLMVSIDQLLDSMKEIGSNCLNNEFNFFKRHICDANKEGMFLFRAARKLRQFLKMNSTGDFDLHLLKVSEGTTILLNCTGQVKGRKPAALGEAQPTKSLEENKSLKEQKKLNDLCFLKRLLQEIKTCWNKILMGTKEH
Scientific Category:
Cytokine/Growth Factors
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
