Bpsbioscience/Human Interleukin-3/90188-A/2 µg
商品编号：
90188-A
品牌：
bpsbioscience
市场价：
¥2600.00
美元价：
1560.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:15kDa
Genbank#:P08700
a.a:20–152
Background:
IL-3isproducedmainlybyT-cellsfollowingcellactivationbyantigensandmitogens,butalsobykeratinocytes,NK-cells,mastcells,endothelialcells,andmonocytes.Theanalysisofbacterial-derivedrecombinantIL-3showsthatglycosylationisnotrequiredfortheactivityofIL-3.IL-3sequencesareevolutionarilynotwellconserved.HumanandmurineIL-3showapproximately29%homologyattheproteinlevelwhilemurineandratIL-3showapproximately54%homology.IL-3-alphaandIL-3-betaaretwoisoformsofratIL-3.IL-3receptorsareexpressedonmacrophages,mastcells,eosinophils,megakaryocytes,basophils,bonemarrowProgenitorcells,andvariousmyeloidleukemiacells.IL-3/IL-3receptorcomplexeshaveaKdsof10-9-10-10M.BindingofIL-3toitsreceptorcausesspecificphosphorylationofa150kDamembraneglycoprotein.
Description:
RecombinantIL-3isadisulfide-linkedmonomericproteinconsistingof134aminoacidresidues,andmigratesasanapproximately15kDaproteinundernon-reducingconditionsandreducingconditionsinSDS-PAGE.OptimizedDNAsequenceencodingHumanInterleukin-3maturechainwasexpressedinE.coli.
UniProt
P08700
Synonym(s):
IL-3,interleukin3,MastCellGrowthFactor,MCGF,IL3
Purity:
≥97%bySDS-PAGEandHPLC
EndotoxinLevel:
<0.1ng/µg(1EU/µg),usingtheLALgelclotmethod.
BIOLOGicalActivity:
TheED50wasdeterminedbythedose-dependentproliferationofHumanTF-1cellswasfoundlessthan0.1ng/ml.
Formulation:
Lyophilizedfroma0.2µmfilteredPBSsolution,pH7.0.
Format:
lyophilizedprotein
Reconstitution:
Reconstituteat0.1-1.0mg/mlindistilledwater.Thissolutioncanthenbedilutedintootherbuffers.Tomaximizeproductcollectionfromvialsurface,vortexbrieflyandthenspindowntorecollecttheliquid.
Storage/StABIlity:
Thelyophilizedproteinisstableforatleast2yearsfromdateofreceiptwhenstoredat-20°C.Uponreconstitution,storeinworkingaliquotsat+4°Cforuptoonemonth,orat-20°Cforuptosixmonths,inthepresenceofacarrierprotein.Avoidrepeatedfreeze/thawcycles.
Reference(s):
1.Mol.Cell.Biol.,Apr2009,29:1682-1693.
2.J.Immunol.,Jan2009,182:1050-1060.
3.J.Immunol.,Feb2009,182:2432-2438.
2.J.Immunol.,Jan2009,182:1050-1060.
3.J.Immunol.,Feb2009,182:2432-2438.
Warning(s):
Avoidfreeze/thawcycles
AminoAcidSequence:
APMTQTTPLKTSWVNCSNMIDEIITHLKQPPLPLLDFNNLNGEDQDILMENNLRRPNLEAFNRAVKSLQNASAIESILKNLLPCLPLATAAPTRHPIHIKDGDWNEFRRKLTFYLKTLENAQAQQTTLSLAIF
ScientificCategory:
Cytokine/GrowthFactors
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
