Bpsbioscience/PD-1:PD-L1 Homogeneous Assay Kit/72014/384 reactions
商品编号：
72014
品牌：
bpsbioscience
市场价：
¥21400.00
美元价：
12840.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:
ThePD-1:PD-L1HomogeneousAssayKitisdesignedtomeasuretheinhibitionofPD-1bindingtoPD-L1.Withthiskit,onlythreesimplestepsonamicrotiterplatearerequired.First,asamplecontainingPD-1andaninhibitorofchoiceisincubatedwiththebiotinylatedPD-L1for60minutes.Next,acceptorbeadsareadded,thendonorbeads,followedbyreADIngtheAlpha-counts.
Synonym(s):
Programmedcelldeath1,PDCD1,PD-1,PD1,SLEB2,CD279,HPD-L,ProgrammedCellDeath1Ligand1,CD274andB7homolog1(B7-H1),PDCD1ligand1,programmeddeathligand1,PDCD1L1
SuppliedAs:
ThePD-1:PD-L1HomogeneousAssayKitcomesinaconvenientAlphaLISA®formatwithpurifiedbiotinylatedPD-L1,FLAG-taggedPD-1,andassaybuffertoperformatotalof384reactions.
Contraindications:
OnlylimitedamountsofDMSOcanbeincluded,asithasbeenshowntodisruptPD-1-PD-L1interaction.AvoidgreenandbluedyesthatabsorblightintheAlphaScreensignalemissionrange(520-620nm),suchasTrypanBlue.Avoidtheuseofthepotentsingletoxygenquencherssuchassodiumazide(NaN3)ormetalions(Fe2+,Fe3+,Cu2+,Zn2+andNi2+).Thepresenceof>1%RPMI1640culturemediumleadstoasignalreductionduetothepresenceofexcessbiotinandironinthismedium.MEM,whichlacksthesecomponents,doesnotaffectAlphaScreenassays.
Format:
COMPONENTS:
Instructionsforuse:
Seeproductdatasheetfordetailedassayprotocol.
Storage/StABIlity:
Atleastoneyearfromdateofreceiptwhenstoredasdirected.
Application(s):
UsefulforscreeningforinhibitorsofPD-1bindingtoPD-L1
Reference(s):
1.Lin,D.,etal.ProcNatlAcadSciU.S.A.2008,105:3011-3016.
2.Keir,M.E.etal.Annu.Rev.Immunol.2008,26:677-704.
2.Keir,M.E.etal.Annu.Rev.Immunol.2008,26:677-704.
Notes:
MATERIALSORINSTRUMENTSREQUIREDBUTNOTSUPPLIED:
AlphaLISAFLAGacceptorbeads,5mg/ml(Perkinelmer#AL112C)
AlphaScreenStreptavidin-conjugateddonorbeads,5mg/ml(PerkinElmer#6760002S)
Optiplate-384(PerkinElmer#6007290)
AlphaScreenmicroplatereader
Adjustablemicropipettorandsteriletips
AlphaLISAFLAGacceptorbeads,5mg/ml(Perkinelmer#AL112C)
AlphaScreenStreptavidin-conjugateddonorbeads,5mg/ml(PerkinElmer#6760002S)
Optiplate-384(PerkinElmer#6007290)
AlphaScreenmicroplatereader
Adjustablemicropipettorandsteriletips
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Immunotherapy
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
