Bpsbioscience/PCSK9-LDLR TR-FRET Assay Kit/72010/384 reactions
商品编号:
72010
品牌:
bpsbioscience
市场价:
¥14900.00
美元价:
8940.00
产品分类:
其它检测试剂盒
公司分类:
Other_kits
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Background:
PCSK9isacrucialplayerintheregulationofplasmacholesterolhomeostasis.Itbindstotheectodomainofhepaticlow-densitylipidreceptorfamilymembers:lowdensitylipoproteinreceptor(LDLR),verylowdensitylipoproteinreceptor(VLDLR),apolipoproteinEreceptor(LRP1/APOER)andapolipoproteinreceptor2(LRP8/APOER2),andpromotestheirdegradation.PCSK9actsviaanon-proteolyticmechanismtoenhancethedegradationofthehepaticLDLRthroughaclathrinLDLRAP1/ARH-mediatedpathway.MaypreventtherecyclingofLDLRfromendosomestothecellsurfaceordirectittolysosomesfordegradation
Description:
ThePCSK9/LDLRTR-FRETAssayKitisdesignedtomeasuretheinhibitionofPCSK9bindingtoLDLRinahomogeneous384reactionformat.ThisFRET-basedassayrequiresnotime-consumingwashingsteps,makingitespeciallysuitableforhighthroughputscreeningapplications.Theassayprocedureisstraightforwardandsimple;asamplecontainingeuropium-labeled(Eu)LDLRectodomain,dye-labeledacceptor,biotin-labeledPCSK9,andaninhibitorisincubatedfortwohours.Then,thefluorescenceintensityismeasuredusingafluorescencereader.
UniProt
Q8NBP7
Synonym(s):
pcsk9,cholesterol,LDL,kexin
SuppliedAs:
KitcomesinaconvenientformatwithpurifiedATAD2A,ligands,Tbdonor,dye-labeledacceptor,assaybufferandmicrotiterplatetoperform384reactions.
Format:
COMPONENTS:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
Atleast6monthsfromdateofreceiptwhenstoredasdirected.
Application(s):
GreatforscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Notes:
MATERIALSORINSTRUMENTSREQUIREDBUTNOTSUPPLIED:
FluorescentmicroplatereadercapableofmeasuringTimeResolvedFluorescenceResonanceEnergyTransfer(TR-FRET)
Adjustablemicropipettorandsteriletips
FluorescentmicroplatereadercapableofmeasuringTimeResolvedFluorescenceResonanceEnergyTransfer(TR-FRET)
Adjustablemicropipettorandsteriletips
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
CholesterolRelated
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。