RedfluorescentproteinclonedfromDiscosomacoral(DsRedordrFP583)isanimportanttoolinbiotechnologyandcellBIOLOGyasaspectrallydistinctcompanionorsubstituteforthegreenfluorescentprotein(GFP;fromAequoreajellyfish).
AffinitypurifiedAnti-RedFluorescentProtein(RFP),cloneRF5R,MouseIgG1.RecognizesnativeanddenaturedformsofRFPanditsvariants:tag-RFP,turbo-RFP,DeRed,mCherry,mOrange,etc.Purifiedfrommouseascitesfluid.Applications:WesternBlot(1:1000-3000),DotBlot,ELISA,Immunocytochemistry(1:500-2000),Immunoprecipitation.Optimaldilutions/concentrationsshouldbedeterminedbytheenduser.PrecitedM.W.ofRFPbyWesternblotis~26kDa.
1mg/mlin10mMPBS,pH7.2,with0.05%NaN3
Storeat–20°Cforupto2years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze/thawcycles.Aliquotsmaybestoredat+4°Cfor1-2weeks.
