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当前位置: 首页 > 产品中心 > Routine_cell_strain > Bpsbioscience/PD-1:PD-L1 Cell-Based Inhibitor Screening Assay Kit/79377/96 reactions
商品详细Bpsbioscience/PD-1:PD-L1 Cell-Based Inhibitor Screening Assay Kit/79377/96 reactions
Bpsbioscience/PD-1:PD-L1 Cell-Based Inhibitor Screening Assay Kit/79377/96 reactions
Bpsbioscience/PD-1:PD-L1 Cell-Based Inhibitor Screening Assay Kit/79377/96 reactions
商品编号: 79377
品牌: bpsbioscience
市场价: ¥11900.00
美元价: 7140.00
产地: 美国(厂家直采)
公司:
产品分类: 常规细胞株
公司分类: Routine_cell_strain
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Background:
ProgrammedCellDeathProtein1(PD-1)isareceptorwhichisexpressedonactivatedT-cells.BindingofPD-1toitsligands,PD-L1andPD-L2,negativelyregulatesimmuneresponses.ThePD-1ligandsarefoundonmostcancers,andPD-1:PD-L1/2interactioninhibitsTcellactivityandallowscancercellstoescapeimmunesurveillance.
Description:

ThePD-1/PD-L1InhibitorScreeningCell-BasedAssayisabioluminescentcell-basedassaythatcanbeusedtoscreenandprofileinhibitorsofthePD-1:PD-L1interaction.Theassayconsistsoftwomaincomponents:

•Growth-ArrestedPD-1Effectorcells(PD-1/NFATreporter-Jurkatcells):ReporterJurkatTcellsexpressingfireflyluciferasegeneunderthecontrolofNFATresponseelementsandalsoconstitutivelyexpressinghumanPD-1.Thesecryopreservedcellsareprovidedinathaw-anduseformatthatdoesnotrequirecellpropagation.Thesecellsaremodifiednottobeexpandedandareintendedtobeusedinasingleexperiment.

•ExpressionvectorsforTCRactivator,andHumanPD-L1:Transfection-readyvectorsareusedtotransfectcellstocreatethetargetcellsthatoverexpressPD-L1andanengineeredcellsurfaceTcellreceptor(TCR)activator.

MaterialsRequiredButNotProvided:

•HEK293cellanditsgrowthmedium(Thawmedium1,#60187)orothercelllines

•Transfectionreagentformammaliancellline[WeuseLipofectamine™2000(Lifetechnologies#11668027).However,othertransfectionreagentsworkequallywell.]

•Opti-MEMIReducedSerumMedium(Lifetechnologies#31985-062)

•96-welltissueculture-treatedwhiteclear-bottomassayplate

•Luminometer

Synonym(s):
ProgrammedCellDeath1Ligand1,PDL1,PD-L1,B7homolog1,B7H1,B7-H1,CD274,PDCD1L1
SuppliedAs:
Eachkitcontainssufficientreagentsfor100assaysusinga96-wellplate.
Format:

COMPONENTS:

Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
Storecelllineinliquidnitrogenimmediatelyuponreceipt.Storeothercomponentsasdescribedbelow.Stableforatleast6monthsfromdateofreceiptwhenstoredasdirected.
Application(s):

•ScreenforactivatorsorinhibitorsofPD-1signalinginacellularcontext

•CharacterizetheBIOLOGicalactivityofPD-1anditsinteractionswithligands

Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Immunotherapy
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。