HumanTGF-β1(R+DSystems#240-B-002,)
ThawMedium1(BPSCat#60187)
Growthmedium:ThawMedium1 (BPSCat#60187)+G418500µg/ml.
Assaymedium:DMEMmedium(Hyclone,#SH30243.01)+1%BSA+1%Pen/Strep
96-welltissueculturetreatedwhiteclear-bottomassayplate(Corning,#3610)
ONE-STEP™LuciferaseAssaySystem(BPSCat#60690)orotherluciferasereagentsformeasuringfireflyluciferaseactivity
Luminometer

ThawMedium1(BPSCat.#60187):MEMmedium(Hyclone#SH30024.01)supplementedwith10%FBS(Invitrogen#26140-079),1%non-essentialaminoacids(Hyclone#SH30238.01),1mMNapyruvate(Hyclone#SH30239.01),1%Penicillin/Streptomycin(HycloneSV30010.01)

CompleteGrowthMedium:ThawMedium1(BPSCat.#60187)and500µg/mlofGeneticin(Invitrogen,#11811031).

Abe,M.etal.(1994)Anassayfortransforminggrowthfactor-βusingcellstransfectedwithaplasminogenactivatorinhibitor-1promoter-luciferaseconstruct.AnalyticalBiochemistry216:276-584.

Khan,S.etal.(2012)Quantificationofactiveandtotaltransforminggrowthfactor-βlevelsinserumandsolidorgantissuesbybioassay.BMCResearchNotes5:636

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