Bpsbioscience/IDO1，His-Avi标签，生物素标记/79081/50μg
商品编号：
79081
品牌：
bpsbioscience
市场价：
¥8700.00
美元价：
5220.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:48kDa
Genbank#:NM_002164
Tag(s):His-Avi-Tag
a.a:2-403
Description:
HumanIDO1,alsoknownasIndoleamine2,3-dioxygenase1,GenBankAccessionNo.NM_002164,a.a.2-403(end)withanN-terminalHis-Avi-Tag,expressedinanE.colicellexpressionsystem.MW=48kDa.
UniProt
P14902
Synonym(s):
Indoleamine2,3-dioxygenase1,IDO1,IDO1,Indoleamine2,3-Dioxygenase1
AssayConditions:
TheassaywasperformedbyUVabsorptionusingarecombinantIDO1orTDO2andL-Tryptophansubstrate.TheUVabsorptionsignalat321nmiscorrelatedwiththeamountofN-formylkynureninereactionproductofreaction.Allofthereactionswereconductedatroomtemperature.The100µlreactionmixtureinIDOorTDOAssayBuffercontains~40nMIDO1(200ng/wellfor71182or210ng/wellfor79081)orvaryingconcentrations(0-320nM)ofIDO1,900tryptophan,andthecoupledreactioncomponents.Thereactionmixtureincubatedfor165minrespectivelywhilereADIngtheUVabsorptionsignal.Forthenegativecontrol(blank),assaybufferwasaddedinsteadoftheenzyme.AbsorptionsignalsweremeasuredusingaTecanInfiniteM1000platereader.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,0.04%Tween20,20%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
Atleast6monthsat-80°C
Application(s):
Usefulforstudyingenzymekinetics,substratespecificity,andscreeninginhibitors.
Reference(s):
1.Lob,S.etal.,CancerImmunol.Immunother.2009;58(1):153-157.
2.Liu,X.etal.,Blood.2010;115(17):3520-3530.
3.Flick,H.E.,etal.,Int.Nat.J.ofTryptophanRes.2013;6:35-45.
Notes:
Theaggregationstateofthisproducthasbeenconfirmedtobe<10%.
Warning(s):
Avoidfreeze/thawcycles.Storingdilutedproteinisnotrecommended,ifnecessary,usecarrierprotein(BSA0.1–0.5%).
ProductType:
Protein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
