Bpsbioscience/UEV1A(UBE2V1),标志标签/80325/100µg
商品编号:
80325
品牌:
bpsbioscience
市场价:
¥5800.00
美元价:
3480.00
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:20kDa
Genbank#:NM_021988
Tag(s):N-terminalFLAG-tag
a.a:2–170(end)
Description:
UEV1A(ubiquitin-conjugatingenzymeE2variant1),alsoknownasUBE2V1orCROC-1(GenBankAccessionNo.NM_021988),a.a.2-170(end),withN-terminalFLAG-tag,MW=20kDa,expressedinE.coli.
UniProt
Q13404
Synonym(s):
UEV1A,UBE2V1,CROC-1,ubiquitin-conjugatingenzymeE2variant1
AssayConditions:
50μlreactionmix(4mMATP,5mMMgCl2,1mMDTT,UBE1(0.5μg),UEV1AorotherE2,Biotin-Ubiquitin(0.5μg)andSmurf2(2μg)in50mMTris,pH7.4)for30minat37°C.AddtoStreptavidin-coatedplatesfor30minat37°C.Incubatewithanti-Smurf2,thenHRP-labeledsecondaryantibody,andchemiluminescentdetection.
Formulation:
45mMTris-HCl,pH8.0,124mMNaCl,2.4mMKCl,90µg/mlFlagpeptide,10%glycerol,and3mMDTT.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
UsefulinconjunctionwithE1andE3forthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1. ThomsonTM,etal.(1998).FEBSLett.423
(1):49–52.
2. Hau DD, et al. (2006). Biochemistry 45
(32):9866–77.
3. Ma L, et al. (1998). Oncogene 17 (10):
1321–6.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
UbiquitinConjugatingEnzyme(E2)
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。