Bpsbioscience/泛素罗丹明/81151/50µg
商品编号：
81151
品牌：
bpsbioscience
市场价：
¥7400.00
美元价：
4440.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
MW: 8934.2 Da
Genbank #: P62987 (ubiquitin)
Tag(s):
Background:
While the disubstituted rhodamine moiety in Ub-Rho110-G is essentially non-fluorescent, cleavage results in a mono-substituted rhodamine, Rho110-G, which exhibits intense fluorescence when excited at 485 nm. The longer excitation and emission wavelengths (Ex485 nm, Em535 nm) of the rhodamine fluorophore reduces the risk of artifacts in screens due to autofluorescence, which can make ubiquitin-rhodamine more appropriate than Ubiquitin-AMC for some compound screening and profiling assays.
Description:
Ubiquitin-rhodamine 110 is a quenched, fluorescent substrate for deubiquitylases, especially ubiquitin C-terminal hydrolases. Cleavage of the amide bond between the C-terminal glycine of ubiquitin and rhodamine results in an increase in rhodamine fluorescence at 535 nm (Exc. 485 nm).
UniProt
P09936
Synonym(s):
Ub-Rho
Purity:
≥95% by RP-HPLC.
Biological Activity:
Rhodamine-N-term-ubiquitin gives a strong signal in the range of 0.1-1 ?M, depending on exact experimental conditions.
Assay Conditions:
Release of rhodamine fluorescence by UCH enzymes can be monitored using Ex485 nm and Em535 nm wavelengths, respectively.
Solubility:
Soluble in DMSO (10 mg/ml) or aqueous buffers
Format:
Lyophilized solid
Storage / Stability:
Store in the dark at or below –80°C. Stable as supplied for up to 1 year when stored dessicated at –80°C. Store DMSO solutions at –80°C for up to 1 month. Avoid freeze/thaw cycles of solutions.
Application(s):
Useful for assaying ubiquitin C-terminal hydrolytic activity, for studying the activity and specificity of ubiquitin and ubiquitin-like hydrolases, and for screening for modulators of deubiquitylase activity.
Reference(s):
1. Tirat, A., et al. 2005. Synthesis and characterization of fluorescent ubiquitin derivatives as highly sensitive substrates for the deubiquitinating enzymes UCH-L3 and USP-2. Anal. Biochem. 343, 244-255.
2. Hassiepin, U., et al. 2007. A sensitive fluorescence intensity assay for deubiquitinating proteases using ubiquitin-rhodamine 110-glycine as substrate. Anal. Biochem. 371, 201-207.
2. Hassiepin, U., et al. 2007. A sensitive fluorescence intensity assay for deubiquitinating proteases using ubiquitin-rhodamine 110-glycine as substrate. Anal. Biochem. 371, 201-207.
Warning(s):
Protect from light
Amino Acid Sequence:
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
Scientific Category:
Ubiquitination Substrate
Product Type:
Substrate
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
