Bpsbioscience/泛素AMC/81150/50µg
商品编号：
81150
品牌：
bpsbioscience
市场价：
¥7000.00
美元价：
4200.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
MW:8722Da
Genbank#:P62987(ubiquitin)
Tag(s):
Background:
Ubiquitin-AMCispreparedviatheconjugationoftheC-terminusofmature,Ubiquitinwith7-amino-4-methylcoumarin(AMC).ThisconjugationquenchestheintrinsicfluorescenceofAMC.ThehighpurityofBPS"sUbiquitin-AMCcomparedtothatofothercommercialvendorsleadstoagreatersignal:background.
Description:
Ubiquitin-AMCisafluorogenicsubstrateforubiquitinhydrolasesbasedontheC-terminusderivatizationofubiquitinwith7-amido-4-methylcoumarin(AMC).UponincubationwithaproteaserecognizingUbiquitin,suchasUSP2orUCHL3,AMCisreleasedandtheincreaseinfluorescencecanbemeasuredusingEx380nmandEm460nmwavelengths.Thisproteincontainsnoextraneoustags.
UniProt
P09936
Synonym(s):
Ub-AMC
Purity:
≥95%byHPLC.
BIOLOGicalActivity:
TypicalenzymeconcentrationsforUCH-L3are10-100pMandforIsopeptidase-Tare10-100nM.
AssayConditions:
ReleaseofAMCfluorescencebyUCHenzymescanbemonitoredusingEx380nmandEm460nmwavelengths,respectively.
Solubility:
SolubleinDMSOoraqueousbuffers(>1mg/ml)
Format:
Lyophilizedsolid
Storage/StABIlity:
Storeinthedarkatorbelow–80°C.Stableassuppliedforupto1yearwhenstoreddessicatedat–80°C.StoreDMSOsolutionsat–80°Cforupto1month.Avoidfreeze/thawcyclesofsolutions.
Application(s):
Usefulasasubstratefordeubiquitinatingenzymes(DUBs),includingubiquitinC-terminalhydrolases(UCHs)andubiquitinspecificproteases(USPs),aswellasinvestigationofdeconjugatingenzymesubstratespecificityandforscreeningformodulatorsof
Reference(s):
1.Dang,L.C.,etal.KineticandmechaNISTicstudiesonthehydrolysisofubiquitinC-terminal7-amido-4-methylcoumarinbydeubiquitinatingenzymes.Biochemistry,37,1868-1879(1998).
2.Mason,D.E.,etal.Substrateprofilingofdeubiquitinhydrolaseswithapositionalscanninglibraryandmassspectrometry.Biochemistry,43,6535-44(2004).
2.Mason,D.E.,etal.Substrateprofilingofdeubiquitinhydrolaseswithapositionalscanninglibraryandmassspectrometry.Biochemistry,43,6535-44(2004).
Warning(s):
Protectfromlight
AminoAcidSequence:
MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
ScientificCategory:
UbiquitinationSubstrate
ProductType:
Substrate
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
