Bpsbioscience/UBE3A, His-FLAG-tags/80302/20 µg
商品编号：
80302
品牌：
bpsbioscience
市场价：
¥7400.00
美元价：
4440.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 99.8 kDa
Genbank #: NM_130838
Tag(s): N-terminal His-FLAG-tags
a.a: 2–end
Description:
Human UBE3A (var1) (GenBank Accession No. NM_130838), a.a. 2-end, with N-terminal His-FLAG-tag, MW=99.8 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q05086
Synonym(s):
UBE3A, E6AP
Assay Conditions:
50 µl of reaction mixture 4 mM ATP, 5 mM
MgCl2, 1 mM DTT, E1 (0.5 µg), E2 (2.8 µg),
Biotinylated Ubiquitin (1 µg) in 50 mM
Tris pH 7.4 was incubated for 30 min at
37°C. Transfer the reaction mixture to a streptavidin-coated plate and incubate for 30 min at 37°C. Add antibody against UBE3A, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.
Formulation:
40 mM Tris-HCl, pH 8.0,
110 mM NaCl, 2.2 mM KCl, 80 µg/ml FLAG
peptide, 20% Glycerol and 3 mM DTT.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Dhe-Paganon S., et al. Biomol NMR. 51(1-2):185-90. (2011).
2. Jung EM, etal. Int J Mol Med. 28(5):697-704. (2011).
2. Jung EM, etal. Int J Mol Med. 28(5):697-704. (2011).
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Ubiquitin Activating Enzyme (E1)
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
