Bpsbioscience/UBE1 (UBA1), FLAG-tag/80301/100 µg
商品编号：
80301
品牌：
bpsbioscience
市场价：
¥6100.00
美元价：
3660.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 118 kDa
Genbank #: NM_003334
Tag(s): N-terminal FLAG-tag
a.a: 2–end
Description:
Human UBE1 (GenBank Accession No. NM_003334), a.a. 2-end, with N-terminal FLAG-tag, MW=118 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
P22314
Synonym(s):
UBE1, UBA1
Assay Conditions:
UBE1 (100 ng) was
added to a reaction mixture containing 4
mM ATP, 2 mM DTT, 5 mM MgCl2, 2.8 µg
Ubch7, 0.8 µg Nedd4, and 0.5 µg biotin-Ub
in 50 mM Tris, pH 7.4. Reaction mix was
incubated at 37°C for 30 min., then
transferred into a streptavidin-coated plate
and incubated for an additional 30 min at
37°C. The reaction mix was incubated with
Anti-FLAG for 1 hour, then HRP secondary
Ab for 30 min, followed by
chemiluminescent detection.
Formulation:
40 mM tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 3 mM DTT and 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful in conjunction with E2 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Su Z.L. et al., Leuk. Lymphoma 49 (9), 1821-1822 (2008).
2. Ramser J. et al., Am. J. Hum. Genet. 82 (1), 188-193 (2008).
2. Ramser J. et al., Am. J. Hum. Genet. 82 (1), 188-193 (2008).
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Ubiquitin Activating Enzyme (E1)
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
