Bpsbioscience/UbcH7，他的标签（E。大肠杆菌衍生）/80317/100µg
商品编号：
80317
品牌：
bpsbioscience
市场价：
¥5800.00
美元价：
3480.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: E. coli
MW: 18 kDa
Genbank #: NM_003347
Tag(s): N-terminal His-tag
a.a: 2–end
Description:
UbcH7, also known as UBE2L3 (GenBank Accession No. NM_003347), a.a. 2-end, with N-terminal His-tag, MW = 18 kDa, expressed in an E. coli cell expression system.
UniProt
P68036
Synonym(s):
UbcH7, UBE2L3, ubiquitin-conjugating enzyme E2L3
Assay Conditions:
50 µl reaction mix (4 mM ATP, 5 mM MgCl2, 1 mM DTT, UBE1 as an E1 (0.5 µg), UbcH7, Biotin-Ubiquitin (0.5 µg) and Smurf2 as an E3(2 µg) in 50 mM Tris pH 7.4) for 30 min at 37°C, then add to streptavidin coated plates for 30 min. at 37°C. Incubate with anti-Smurf2, then
Europium-labeled secondary antibody, and detect by time resolved fluorescence (λex=330 nm, λem=620 nm).
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful in conjunction with E1 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Katsanis, N., et al. 1998. Genomics 51: 128- 131.
2. Cook, W.J. et al., 1997. Biochemistry 36 (7): 1621–1627.
Warning(s):
Avoid freeze/thaw cycles
Scientific Category:
Ubiquitin Conjugating Enzyme (E2)
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
