
Bpsbioscience/UbcH6 (UBE2E1), His-tag/80316/100 µg
商品编号:
80316
品牌:
bpsbioscience
市场价:
¥5000.00
美元价:
3000.00
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:22kDa
Genbank#:NM_003341
Tag(s):N-terminalHis-tag
a.a:2–end
Description:
UbcH6,alsoknownasUBE2E1(GenBankAccessionNo.NM_003341),a.a.2-end,withN-terminalHis-tag,MW=22kDa,expressedinanE.colicellexpressionsystem.
UniProt
P51965
Synonym(s):
UbcH6,UBE2E1
AssayConditions:
50µlreactionmix(4mMATP,5mMMgCl2,1mMDTT,UBE1asanE1(0.5µg),UbcH6,Biotin-Ubiquitin(0.5µg)andSmurf2asanE3(2µg)in50mMTrispH7.4)for30minat37°C,thenaddtostreptavidincoatedplatesfor30min.at37°C.Incubatewithanti-Smurf2,thenEuropium-labeledsecondaryantibody,anddetectbytimeresolvedfluorescence(λex=330nm,λem=620nm).
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and200mMimidazole
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
UsefulinconjunctionwithE1andE3forthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Sommer,T.,etal.1993.Nature365(6442):176-9.
2.Yang,M.etal.1997.J.Biol.Chem.272:1970-1975.
2.Yang,M.etal.1997.J.Biol.Chem.272:1970-1975.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
UbiquitinConjugatingEnzyme(E2)
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。