Bpsbioscience/SMURF1, FLAG-tag/80402/20 µg
商品编号：
80402
品牌：
bpsbioscience
市场价：
¥7400.00
美元价：
4440.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:70kDa
Genbank#:NM_020429
Tag(s):N-terminalFLAG-tag
a.a:150–end
Description:
HumanSMURF1(GenBankAccessionNo.NM_020429),a.a.150-end,withN-terminalFLAG-tag,MW=70kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
UniProt
Q9HCE7
Synonym(s):
SMADspecificE3ubiquitinproteinligase1,SMURF1
AssayConditions:
10uLofreactionmixturecontains50mMTris,pH7.4,5mMMgCl2,0.01%Tween,1mMDTT,0.2µMUb[Biotin],50nMUbe1,1mMATP,0.25µMofUBCH5b,andvariousamountofsmurf1.ThereactionwasinitiatedwiththeadditionofATP,andincubatedat30Cfor3hours.Tblabeledanti-Flagantibodydonorandxl665labeledacceptorwasaddedandincubatedatroomtemperatureforhour.
Formulation:
40mMTris-HClpH8.0,110mMNaCl,2.2mMKCl,0.04%Tween-20,3mMDTT,and20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
Atleast6monthsat–80°C.Avoidfreeze/thawcycles.Proteinmaybedilutedto≥100µg/mlinPBS+glycerolandstoredat-80°C.
Application(s):
UsefulinconjunctionwithE1andE2forthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.NarimatsuM.etal.,Cell137(2),295-307(2009)
2.FukunagaE.etal.,J.Biol.Chem.283(51),35660-35667(2008).
2.FukunagaE.etal.,J.Biol.Chem.283(51),35660-35667(2008).
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
UbiquitinE3ligase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
