His标签/SBICENP2/81095
商品编号：
81095
品牌：
bpsbioscience
市场价：
¥39400.00
美元价：
23640.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: E. coli
MW: 28 kDa
Tag(s): N-terminal His-tag
Background:
SUMO Protease 2, a highly active and robust recombinant protease, cleaves hSUMO3 from recombinant fusion proteins. Unlike thrombin, EK, or TEV protease, which recognize short, linear sequences, SUMO Protease 2 recognizes the tertiary structure of huSUMO3. As a result, SUMO Protease 2 will not cleave within the fused protein of interest.
Description:
Human SENP2 recombinant protein, with N-terminal His-tag, expressed in E. coli.
UniProt
Q9HC62
Synonym(s):
SUMO Protease 2
Purity:
≥95% by SDS-PAGE and RP-HPLC
Supplied As:
10U/µl in aqueous buffer
Specific Activity:
≥105 Units/mg
Unit Definition:
1 Unit of SUMO Protease 2 cleaves >90% of 100 µg SUMO Protease 2 Control Protein in 1 hour (37°C)
Formulation:
50 mM HEPES, pH 7.5, 150 mM NaCl, 10% glycerol
Format:
Aqueous buffer solution
Storage / Stability:
Store at or below –80°C. Stable as supplied for up to 1 year when stored dessicated at –80°C.
Application(s):
Useful for removal of SUMO-tags from recombinant SUMO fusion proteins
Reference(s):
1. MarblestoneJG, et al. (2006). "Comparison of SUMO fusion technology with traditional gene fusion systems: Enhanced expression and solubility with SUMO," Protein Science, 15:182-9.
2. Butt TR, Edavettal SC, Hall JP, Mattern MR (2005). "SUMO fusion technology for difficult-to-express proteins," Protein Expr Purif., 43(1):1-9.
3. Zuo X, et al. (2005). "Enhanced expression and purification of membrane proteins by SUMO fusion in Escherichia coli.," J. Struct. Funct. Gen., 6:103-11.
4. Malakhov MP, et al. (2004). "SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins," J. Struct. Funct.Gen., 5:75-86.
2. Butt TR, Edavettal SC, Hall JP, Mattern MR (2005). "SUMO fusion technology for difficult-to-express proteins," Protein Expr Purif., 43(1):1-9.
3. Zuo X, et al. (2005). "Enhanced expression and purification of membrane proteins by SUMO fusion in Escherichia coli.," J. Struct. Funct. Gen., 6:103-11.
4. Malakhov MP, et al. (2004). "SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins," J. Struct. Funct.Gen., 5:75-86.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Ubiquitination (SUMOylation)
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
