
Bpsbioscience/SENP2, His-tag/81096/500 units
商品编号:
81096
品牌:
bpsbioscience
市场价:
¥9300.00
美元价:
5580.00
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:E.coli
MW:28kDa
Tag(s):N-terminalHis-tag
Background:
SUMOProtease2,ahighlyactiveandrobustrecombinantprotease,cleaveshSUMO3fromrecombinantfusionproteins.Unlikethrombin,EK,orTEVprotease,whichrecognizeshort,linearsequences,SUMOProtease2recognizesthetertiarystructureofhuSUMO3.Asaresult,SUMOProtease2willnotcleavewithinthefusedproteinofinterest.
Description:
HumanSENP2recombinantprotein,withN-terminalHis-tag,expressedinE.coli.
UniProt
Q9HC62
Synonym(s):
SUMOProtease2
Purity:
≥95%bySDS-PAGEandRP-HPLC
SuppliedAs:
10U/µlinaqueousbuffer
SpecificActivity:
≥105Units/mg
UnitDefinition:
1UnitofSUMOProtease2cleaves>90%of100µgSUMOProtease2ControlProteinin1hour(37°C)
Formulation:
50mMHEPES,pH7.5,150mMNaCl,10%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
Storeatorbelow–80°C.Stableassuppliedforupto1yearwhenstoreddessicatedat–80°C.
Application(s):
UsefulforremovalofSUMO-tagsfromrecombinantSUMOfusionproteins
Reference(s):
1.MarblestoneJG,etal.(2006)."ComparisonofSUMOfusiontechnologywithtrADItionalgenefusionsystems:EnhancedexpressionandsolubilitywithSUMO,"ProteinScience,15:182-9.
2.ButtTR,EdavettalSC,HallJP,MatternMR(2005)."SUMOfusiontechnologyfordifficult-to-expressproteins,"ProteinExprPurif.,43(1):1-9.
3.ZuoX,etal.(2005)."EnhancedexpressionandpurificationofmembraneproteinsbySUMOfusioninEscherichiacoli.,"J.Struct.Funct.Gen.,6:103-11.
4.MalakhovMP,etal.(2004)."SUMOfusionsandSUMO-specificproteaseforefficientexpressionandpurificationofproteins,"J.Struct.Funct.Gen.,5:75-86.
2.ButtTR,EdavettalSC,HallJP,MatternMR(2005)."SUMOfusiontechnologyfordifficult-to-expressproteins,"ProteinExprPurif.,43(1):1-9.
3.ZuoX,etal.(2005)."EnhancedexpressionandpurificationofmembraneproteinsbySUMOfusioninEscherichiacoli.,"J.Struct.Funct.Gen.,6:103-11.
4.MalakhovMP,etal.(2004)."SUMOfusionsandSUMO-specificproteaseforefficientexpressionandpurificationofproteins,"J.Struct.Funct.Gen.,5:75-86.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Ubiquitination(SUMOylation)
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。