Bpsbioscience/Praja1，相扑标签/81055/25µg
商品编号：
81055
品牌：
bpsbioscience
市场价：
¥8400.00
美元价：
5040.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: E. coli
MW: 71 kDa
Genbank #: NP_660095
Tag(s): N-terminal SUMO-tag
a.a: full length
Background:
Praja1 (along with Praja2, 53% identity) is a RING-H2 domain E3 (ubiquitin protein ligase) that is involved in the conjugation of ubiquitin to target substrates along with E1 and E2 enzymes. Substrates for Praja1 include Dlxin-1 (MAGE-D1, human) which regulates Dlx5 dependent transcriptional function (involved in osteoblast differentiation) and ELF and Smad3 involved in the TGF-beta signaling pathway implicated in gastrointestinal cancers.
Description:
Human Praja1 recombinant protein (Genbank Accession No. NP_660095), full length with N-terminal SUMO-tag, expressed in E. coli.
UniProt
Q8NG27
Synonym(s):
RNF70, RING finger protein 70
Purity:
≥85% by SDS-PAGE
Biological Activity:
Typical enzyme concentration of 100 nM - 5 mM is used for in vitro conjugation, depending on conditions.
Formulation:
50 mM Tris Buffer, pH 7.6, 150 mM NaCl, 10% glycerol
Format:
Aqueous buffer solution
Storage / Stability:
Stable for ≥1 year at –80°C.
Application(s):
Useful as an E3 ligase in ubiquitin-conjugating reactions and to analyze upregulation/downregulation of E3 enzymes.
Reference(s):
1. Yu, P., et al. PJA1, encoding a RING-H2 finger ubiquitin ligase, is a novel human X chromosome gene abundantly expressed in brain. Genomics, 2002. 79(6): p. 869-74.
2. Mishra, L., et al. The role of PRAJA and ELF in TGF-beta signaling and gastric cancer. Cancer Biol Ther, 2005. 4(7): p. 694-9.
3. Saha, T., et al. RING finger-dependent ubiquitination by PRAJA is dependent on TGF-beta and potentially defines the functional status of the tumor suppressor ELF. Oncogene, 2006. 25(5): p. 693-705.
4. Sasaki, A., et al., A RING finger protein Praja1 regulates Dlx5-dependent transcription through its ubiquitin ligase activity for the Dlx/Msx-interacting MAGE/Necdin family protein, Dlxin-1. J Biol. Chem, 2002. 277(25): p. 22541-6.
2. Mishra, L., et al. The role of PRAJA and ELF in TGF-beta signaling and gastric cancer. Cancer Biol Ther, 2005. 4(7): p. 694-9.
3. Saha, T., et al. RING finger-dependent ubiquitination by PRAJA is dependent on TGF-beta and potentially defines the functional status of the tumor suppressor ELF. Oncogene, 2006. 25(5): p. 693-705.
4. Sasaki, A., et al., A RING finger protein Praja1 regulates Dlx5-dependent transcription through its ubiquitin ligase activity for the Dlx/Msx-interacting MAGE/Necdin family protein, Dlxin-1. J Biol. Chem, 2002. 277(25): p. 22541-6.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Ubiquitination (Ubiquitin Ligase (E3))
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
