Bpsbioscience/NEDD8 E1, His-tag/81087/50 µg
商品编号：
81087
品牌：
bpsbioscience
市场价：
¥5900.00
美元价：
3540.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: E. coli
MW: 61 kDa (APPBP1), 52 kDa (UBA3)
Tag(s): His-tag (APPBP1), no tag (Uba3)
Background:
The NEDD8 activating E1 enzyme is a heterodimer composed of APPBP1 and UBA3 subunits. The APPBP1/UBA3 enzyme has homology to the N- and C-terminal halves of the ubiquitin E1 enzyme, respectively. The UBA3 subunit contains the catalytic center and activates NEDD8 in an ATP-dependent reaction by forming a high-energy thiolester intermediate. The activated NEDD8 is subsequently transferred to the UbcH12 E2 enzyme, and is then conjugated to specific substrates in the presence of the appropriate E3 ligases.
Description:
Human NEDD8 E1 recombinant protein complex, with His-tagged APPBP1 and untagged Uba3, co-expressed in E. coli.
UniProt
Q8TBC4
Synonym(s):
Nedd8, APPBP1/Uba3
Purity:
≥80%
Biological Activity:
1–100 nM is used for the in vitro conjugation
Formulation:
50 mM Tris Buffer, pH 8.0, 150 mM NaCl, 10% glycerol
Format:
Aqueous buffer solution
Storage / Stability:
Store at or below –80°C. Stable as supplied for up to 1 year when stored dessicated at –80°C.
Application(s):
Useful for NEDD8 conjugation reactions and NEDDylation pathway studies
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Ubiquitination (Ubiquitin Activating Enzyme (E1))
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
