Bpsbioscience/MuRF3, SUMO-His-tags/81054/25 µg
商品编号：
81054
品牌：
bpsbioscience
市场价：
¥7100.00
美元价：
4260.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: E. coli
MW: 40.3 kDa (without SUMO tag)
Genbank #: Q9BYV2
Tag(s): N-terminal SUMO-His-tags
a.a: full length
Background:
MuRF3 is a RING domain E3 ligase that is involved in the conjugation of ubiquitin to target substrates. MuRF3 has been demonstrated to function with the E2 enzyme UBE2D3 (UbcH5c) in vitro. MuRF3 is also known as TRIM54 (tripartite motif containing 54) containing a RING-finger/B-box/coiled-coil tripartite fold. MuRF3 has been implicated along with MuRF1 as regulators of protein degradation in striated muscle. MuRF3 also plays important roles in maintaining cardiac function after myocardial infarction.
Description:
Human MuRF3 recombinant protein (Genbank Accession No. Q9BYV2), full length with N-terminal SUMO- and His-tags, expressed in E. coli.
UniProt
Q9BYV2
Synonym(s):
E3 ubiquitin-protein ligase TRIM54, MURF3, MuRF-3, MURF-3, Muscle-specific RING finger protein 3, RING finger protein 30, RNF30, Tripartite motif-containing protein 54
Purity:
≥90% by SDS-PAGE
Biological Activity:
Typical enzyme concentration of 20-1000 nM is used for in vitro conjugation depending on assay conditions.
Formulation:
50 mM Tris Buffer, pH 7.6, 150 mM NaCl, 10% glycerol
Format:
Aqueous buffer solution
Storage / Stability:
Stable for ≥1 year at –80°C.
Application(s):
Useful as an E3 ligase in ubiquitin-conjugating reactions and to analyze upregulation/downregulation of E3 enzymes.
Reference(s):
1. Balasubramanian, S., et al. Enhanced ubiquitination of cytoskeletal proteins in pressure overloaded myocardium is accompanied by changes in specific E3 ligases. J. Mol. Cell Cardiol., 2006. 41(4): p. 669-79.
2. Fielitz, J., et al. Myosin accumulation and striated muscle myopathy result from the loss of muscle RING finger 1 and 3. J. Clin. Invest., 2007. 117(9): p. 2486-95.
3. Fielitz, J., et al. Loss of muscle-specific RING-finger 3 predisposes the heart to cardiac rupture after myocardial infarction. Proc. Natl. Acad. Sci. USA, 2007. 104(11): p. 4377-82.
2. Fielitz, J., et al. Myosin accumulation and striated muscle myopathy result from the loss of muscle RING finger 1 and 3. J. Clin. Invest., 2007. 117(9): p. 2486-95.
3. Fielitz, J., et al. Loss of muscle-specific RING-finger 3 predisposes the heart to cardiac rupture after myocardial infarction. Proc. Natl. Acad. Sci. USA, 2007. 104(11): p. 4377-82.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Ubiquitination (Ubiquitin Ligase (E3))
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
