Bpsbioscience/MDM2，GST标签/80751/20µg
商品编号：
80751
品牌：
bpsbioscience
市场价：
¥7500.00
美元价：
4500.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:82kDa
Genbank#:NM_002392
Tag(s):N-terminalGST-tag
a.a:2–498
Description:
Mdm2(mousedoubleminute2,humanhomolog,alsoknownasHdm2),GenBankAccessionNo.NM_002392,(2-498)withN-terminalGST-tag,MW=82kDa,expressedinabaculovirusexpressionsystem.
UniProt
Q00987-11
Synonym(s):
Mdm2,mousedoubleminute2,humanhomolog,Hdm2
AssayConditions:
50µlofreactionmix(4mMATP,5mMMgCl2,1mMDTT,E1(0.5µg),E2(2.8µg),Biotin-Ubiquitin(0.5µg)andMdm2(0-0.5µg)in50mMTrispH7.4)wasincubatedfor30min.at37°C.Thereactionmixturewastransferredtoastreptavidin-coatedplateandincubatedforadditional30minat37°C.Anti-Mdm2andHRP-labeledsecondaryantibodywereincubatedwiththereactionmixturefor1houreach,followedbychemiluminescentdetection.
Formulation:
50mMTris-HCl,pH7.5,300mMNaCl,1mMEDTA,1mMDTT,5mMglutathioneand10%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
UsefulinconjunctionwithE1andE2forthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Fang,S.,etal.(2000).J.Biol.Chem.275,8945-8951.
2.Chen,Jetal.(1995).Mol.Med.1,142-152.
2.Chen,Jetal.(1995).Mol.Med.1,142-152.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
UbiquitinE3ligase
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
