Bpsbioscience/TurboTEV Protease/50308/10000 units
商品编号：
50308
品牌：
bpsbioscience
市场价：
¥9000.00
美元价：
5400.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Tobaccoetchvirus(TEV)
HostSpecies:E.coli
MW:52kDa
Description:
TurboTEVProteasecontainsanenhancedformofacatalyticfragmentoftheN1aproteinofTobaccoetchvirus(TEV),acysteineproteasethatrecognizesthecleavagesiteofGlu-Asn-Leu-Tyr-Phe-Gln-GlyandcleavesbetweenGlnandGly.TurboTEVProteaseisarestrictiongradeproteasethathasarobustactivityat4°CwithhighspecificityandgreatstABIlity.Itdoesnotrequireanyspecialbufferforitsactivityandcanbeusedinabuffermostsuitableforthetargetprotein.TurboTEVProteaseisa52kDaproteinwithbothGSTandHis-tagssoitcanbeeasilyremovedbyeitherNi-chelatingorGlutathione(GSH)resinalongwiththecleavedtag.Inpractice,1mg(10,000units)ofTurboTEVProteasecleaves>90%of100mgofacontroltargetproteinat4°Cin16hours.Nonon-specificcleavagehasbeenobservedunderthesameconditionwhenTurboTEVProteaseandthecontroltargetproteinwasmixedat1:10ratio.
UniProt
P04517
Purity:
≥95%
SpecificActivity:
≥10units/µg
UnitDefinition:
Oneunitcleaves>85%of3µgcontrolsubstratein1hourat30°C
AssayConditions:
A49kDaGST-fusionprotein(C)at1mg/mlisincubatedwithTurboTEVorTEVProteaseinabufferof25mMTris-HCl,pH8.0,150mMNaCl,14mMβ-mercaptoethanolat4°Cfor16hours.Thecleavedproductsare27kDaand22kDa.Nonon-specificcleavagehasbeenobservedunderthesameconditionwhenTurboTEVProteaseandthecontroltargetproteinweremixedat1:10ratio.
Concentration:
2mg/ml
Formulation:
25mMTris-HCl,pH8.0,50mMNaCl,1mMTCEP,50%glycerol
Format:
Aqueousbuffersolution
Instructionsforuse:
Inpractice,1mg(10,000units)ofTurboTEVProteasecleaves>90%of100mgofacontroltargetproteinat4°Cin16hours.
Storage/Stability:
>6monthsat–20°C.TurboTEVretains>80%activityafterstorageatroomtemperatureforover65hours.
Application(s):
ImmobilizedTEVproteaseonstreptavidin-agaroseisanefficienttoolforfusionproteincleavage.TEVproteasehasalsobeenusedinastudytoinvestigateproteolyticprocessingofnlrp1bforinflammasomeactivity.
Reference(s):
1.TimmerJC,SalvesenGS.MethodsMolBiol.2011;753:243-55.
2.BradleyCBCFrewetal.2012.PLoSPathogens,8(4),e1002659.
2.BradleyCBCFrewetal.2012.PLoSPathogens,8(4),e1002659.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Protease
ProductType:
Enzyme
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
