Bpsbioscience/Sortase A, S. aureus, His-tag/71086/50 µg
商品编号：
71086
品牌：
bpsbioscience
市场价：
¥9800.00
美元价：
5880.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Staphylococcusaureus
HostSpecies:E.coli
MW:27.1kDa
Genbank#:AF162687
Tag(s):N-terminalHis-tag
a.a:26–206(end)
Background:
StaphylococcalSortaseAisabacterialtranspeptidasethatcovalentlyattachesproteinstothebacterialcellwallbycleavingbetweenthreonineandglycineatanLPXTGrecognitionmotifandcatalyzestheformationofanamidebondbetweenthecarboxyl-groupofthreonineandtheamino-groupofthecell-wallpeptidoglycan.Thischemistryhasbeenexploitedasamolecular"stapler"tosite-specificallylinkproteinswiththeC-terminalLPETGXmotiftootherproteinsormoleculespossessingaglycineoraminomethylenemotif.Thisreactionisapplicabletoproteinsinsolutionandonthelivingcellsurface.
Description:
StaphylococcusaureusSortaseA(GenBankAccessionNo.AF162687),a.a.26–206(end)withN-terminalHis-tag,MW=21.7kDa,expressedinanE.coliexpressionsystem.
UniProt
Q9S446
Synonym(s):
SrtA,sortaseA
AssayConditions:
Assaywasdoneinthereactionbuffer(50µl)containing50mMHEPES(pH=7.4),150mMNaCl,5mMCaCl2,5mM(Gly)3,25mMAbz/Dnpsubstrate,andSortaseAfor30minat30oC.FluorescenceintensitywasmeasuredatEx320nm/Em420nm.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,8mMimidazole,0.04%Tween-20,and20%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C
Application(s):
Suitableforusewithinvitroproteinligationreactions.
Reference(s):
1.Mazmanian,S.K.,etal.,Science.1999Jul30,285(5428):760-3.
2.Kappel,K.,etal.,ProteinSci.2012Dec,21(12):1858-71.
2.Kappel,K.,etal.,ProteinSci.2012Dec,21(12):1858-71.
Warning(s):
Avoidfreeze/thawcycles
ScientificCategory:
Miscellaneous
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
