Bpsbioscience/Sortase A, Octamutant, His-Tag/72518/50 µg
商品编号：
72518
品牌：
bpsbioscience
市场价：
¥9400.00
美元价：
5640.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:S.aureus
HostSpecies:E.coli
MW:22kDa
Genbank#:AF162687
Tag(s):N-terminalHis-tag
a.a:26-206(end)
Background:
StaphylococcalSortaseAisabacterialtranspeptidasethatcovalentlyattachesproteinstothebacterialcellwallbycleavingbetweenthreonineandglycineatanLPXTGrecognitionmotifandcatalyzestheformationofanamidebondbetweenthecarboxyl-groupofthreonineandtheamino-groupofthecell-wallpeptidoglycan.Thischemistryhasbeenexploitedasamolecular"stapler"tosite-specificallylinkproteinswiththeC-terminalLPETGXmotiftootherproteinsormoleculespossessingaglycineoraminomethylenemotif.Thisreactionisapplicabletoproteinsinsolutionandonthelivingcellsurface.
Description:
StaphylococcusaureussortaseAOctamutant(P94R,D124G,D160N,D165A,Y187L,E189R,K190E,K196T),a.a26-206(end)GenBankAccessionNo.AF162687withN-terminalHistag,MW=22kDa,expressedinanE.coliexpressionsystem.
Synonym(s):
SrtA,sortaseA
Formulation:
40mMTrispH8.0,110mMNaCl,2.2mMKCl,400mMimidazoleand20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
Atleast6monthsat–80°C.
Application(s):
Suitableforusewithinvitroproteinligationreactions.
Reference(s):
1.Chen,I.etal.,ProcNatl.Acad.Sci.(USA).2011;108(28):11399-11404.
2.Kappel,K.,etal.,ProteinSci.2012Dec;21(12):1858-71.
2.Kappel,K.,etal.,ProteinSci.2012Dec;21(12):1858-71.
Warning(s):
Avoidfreeze/thawcycles.Storingdilutedenzymeisnotrecommended,ifnecessary,usecarrierprotein(BSA0.1–0.5%).
ScientificCategory:
Other
ProductType:
Recombinantprotein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
