Bpsbioscience/Sortase A Pentamutant, S. aureus, His-tag/71046/50 µg
商品编号:
71046
品牌:
bpsbioscience
市场价:
¥9800.00
美元价:
5880.00
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Species:S.aureus
HostSpecies:E.coli
MW:21.7kDa
Genbank#:AF162687
Tag(s):N-terminalHis-tag
a.a:26-206(end)
Background:
StaphylococcalSortaseAisabacterialtranspeptidasethatcovalentlyattachesproteinstothebacterialcellwallbycleavingbetweenthreonineandglycineatanLPXTGrecognitionmotifandcatalyzestheformationofanamidebondbetweenthecarboxyl-groupofthreonineandtheamino-groupofthecell-wallpeptidoglycan.Thischemistryhasbeenexploitedasamolecular"stapler"tosite-specificallylinkproteinswiththeC-terminalLPETGXmotiftootherproteinsormoleculespossessingaglycineoraminomethylenemotif.TheSortaseAPentamutantexhibitshigheractivitythanthewildtypeprotein.Thisreactionisapplicabletoproteinsinsolutionandonthelivingcellsurface.
Description:
StaphylococcusaureussortaseAPentamutant(P94R,D160N,D165A,K190E,K196T),a.a.26-206(end)GenBankAccessionNo.AF162687withN-terminalHistag,MW=21.7kDa,expressedinanE.coliexpressionsystem.
UniProt
C7TEJ5
Synonym(s):
SrtA,sortaseA,pentamutant
AssayConditions:
Assaywasdoneinthereactionbuffer(50μl)containing50mMHEPES(pH=7.4),150mMNaCl,5mMCaCl2,5mM(Gly)3,25mMAbz/Dnpsubstrate,andSortaseAfor30minat30°C.FluorescenceintensitywasmeasuredatEx320nm/Em420nm.
Formulation:
40mMTrispH8.0,110mMNaCl,2.2mMKCl,400mMimidazoleand20%glycerol
Format:
Aqueousbuffersolution
Storage/StABIlity:
Atleast6monthsat–80°C.
Application(s):
Suitableforusewithinvitroproteinligationreactions.
Reference(s):
1.Chen,I.etal.,ProcNatl.Acad.Sci.(USA).2011;108(28):11399-11404.
2.Kappel,K.,etal.,ProteinSci.2012Dec;21(12):1858-71.
2.Kappel,K.,etal.,ProteinSci.2012Dec;21(12):1858-71.
Warning(s):
Avoidfreeze/thawcycles.Storingdilutedenzymeisnotrecommended,ifnecessary,usecarrierprotein(BSA0.1–0.5%).
ScientificCategory:
Other
ProductType:
Recombinantprotein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。