Bpsbioscience/MMP9 (Q279R), His-tag/80215/20 µg
商品编号：
80215
品牌：
bpsbioscience
市场价：
¥8500.00
美元价：
5100.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:HEK293
MW:77.8kDa
Genbank#:NM_004994
Tag(s):C-terminalHis-tag
a.a:20-707(end)
Description:
Humanmatrixmetallopeptidase-9(MMP9),alsoknownasgelatinaseB,MANDP2,CLG4B,Macrophagegelatinase,GELBandEC3.4.24,withGlntoArgmutationona.a.279,GenBankAccessionNo.NM_004994),a.a.20-707(end)withC-terminalHis-tag,MW=77.8kDa,expressedinanHEK293cellexpressionsystem.
UniProt
P14780
Synonym(s):
matrixmetallopeptidase-9,MMP9,gelatinaseB,MANDP2,CLG4B,Macrophagegelatinase,GELB,EC3.4.24,MMP
SpecificActivity:
≥2500pmole/min/µg
AssayConditions:
Activation:Thisisaproformenzymeandrequiresactivationpriortotestingactivity.Dilutetheenzymeto100ng/mlinasolutioncontaining50mMHEPES,pH7.4,10mMCaCl2,0.05%Brij-35,and1mMAPMA(amino-phenylmercuricacetate).Incubateat37ºCfor2hours.
Assayconditions:Reactionmixturewith10μM390MMPsubstrate1(Anaspec).Incubatefor30minutesatroomtemperature.Fluorescenceintensityismeasuredatexc328/em393.
Assayconditions:Reactionmixturewith10μM390MMPsubstrate1(Anaspec).Incubatefor30minutesatroomtemperature.Fluorescenceintensityismeasuredatexc328/em393.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,200mMimidazole,and20%glycerol.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Wenham,R.M.,etal.,J.Soc.Gynecol.Investig.2003Sep;10(6):381-7.
2.Li,X.,etal.,J.CancerRes.Clin.Oncol.2013Sep;139(9):1433-47.
2.Li,X.,etal.,J.CancerRes.Clin.Oncol.2013Sep;139(9):1433-47.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Protease
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
