Bpsbioscience/MMP1, His-tag/80214/20 µg
商品编号：
80214
品牌：
bpsbioscience
市场价：
¥8500.00
美元价：
5100.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: HEK293
MW: 53 kDa
Genbank #: NM_002421
Tag(s): C-terminal His-tag
a.a: 20-469 (end)
Description:
Human matrix metallopeptidase-1 (MMP1), also known as CLG, Interstitial Collagenase, Fibroblast Collagenase, and EC 3.4.24.7 (GenBank Accession No. NM_002421), a.a. 20-469 (end), with C-terminal His-tag, MW=53 kDa, expressed in an HEK293 cell expression system.
UniProt
P03956
Synonym(s):
matrix metallopeptidase-1 , MMP1, CLG, Interstitial Collagenase, Fibroblast Collagenase, EC 3.4.24.7, MMP
Specific Activity:
≥ 105 pmole/min/µg
Assay Conditions:
Activation: This is a proform enzyme and
requires activation prior to testing activity. Dilute
the enzyme to 100 ng/ml in a solution containing
50 mM HEPES, pH 7.4, 10 mM CaCl2, 0.05%
Brij-35, and 1 mM APMA (amino-phenyl
mercuric acetate). Incubate at 37ºC for 2 hours.
Assay conditions: Reaction mixture with 10 μM 390 MMP substrate 1 (Anaspec). Incubate for 30 minutes at room temperature. Fluorescence intensity is measured at exc328/em393.
Assay conditions: Reaction mixture with 10 μM 390 MMP substrate 1 (Anaspec). Incubate for 30 minutes at room temperature. Fluorescence intensity is measured at exc328/em393.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Wenham, R.M., et al., J. Soc. Gynecol.
Investig. 2003 Sep;10(6):381-7.
2. Li, X., et al., J. Cancer Res. Clin. Oncol. 2013 Sep;139(9):1433-47.
2. Li, X., et al., J. Cancer Res. Clin. Oncol. 2013 Sep;139(9):1433-47.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
Protease
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
