Bpsbioscience/TNKS2组蛋白核糖基化比色测定试剂盒/80583/96反应
商品编号：
80583
品牌：
bpsbioscience
市场价：
¥15500.00
美元价：
9300.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Description:
TheTNKS2HistoneRibosylationColorimetricAssayKitisdesignedtomeasureTankyrase2(TNKS2)activityforscreeningandprofilingapplications.TNKS2catalyzestheNAD-dependentadditionofpoly(ADP-ribose)tothesubstrateproteins.ThekeytotheTNKS2HistoneRibosylationAssayisthebiotinylatedNAD+substrate.Withthiskit,onlythreesimplestepsarerequiredforTNKS2reactions.First,histoneproteinsarecoatedona96-wellplate.Next,thebiotinylatedNAD+substrateisincubatedwithanassaybufferthatcontainstheTNKS2enzyme.Finally,theplateistreatedwithstreptavidin-HRPfollowedbyadditionofthecolorimetricHRPsubstratetoproducecolorthatcanthenbemeasuredusingaUV/Visspectrophotometermicroplatereader.
Synonym(s):
TNKS2,poly(ADP-ribose)polymerase5b,poly(ADP-ribosyl)transferase5b,PARP5B,PARP5C,TRF1-interactingankyrin-relatedADP-ribosepolymerase2,TANK2,tanykyrase-2,TNKL
SuppliedAs:
TheTNKS2assaykitcomesinaconvenient96-wellformat,withpurifiedTNKS2enzyme,histonemixture,andPARPassaybufferfor100enzymereactions.
Format:
COMPONENTS:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol
Storage/StABIlity:
Stableatleast6monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
Brown,J.A.,Marala,R.B.J.Pharmacol.Toxicol.Methods200247:137-41.
ApplicationReference(s):
1. SmallMoleculesInspiredbytheNaturalProductWithanolidesasPotentInhibitorsofWntSignaling (2017)
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
PARP
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
