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当前位置: 首页 > 产品中心 > Other_kits > Bpsbioscience/PARPtrap™ Assay Kit/80584/96 reactions
商品详细Bpsbioscience/PARPtrap™ Assay Kit/80584/96 reactions
Bpsbioscience/PARPtrap™ Assay Kit/80584/96 reactions
Bpsbioscience/PARPtrap™ Assay Kit/80584/96 reactions
商品编号: 80584
品牌: bpsbioscience
市场价: ¥22600.00
美元价: 13560.00
产地: 美国(厂家直采)
公司:
产品分类: 其它检测试剂盒
公司分类: Other_kits
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Background:
PARP1isknowntobinddamagedDNAthroughitsN-terminalzincfingerdomain.AfterPARP1ribosylatesitself(autoribosylation),itdissociatesfromDNAduetotheaccumulatednegativechargeoftheribosylpolymer.TrappedPARP-DNAcomplexeshavebeenshowntobecytotoxictocancercells.
Description:

ThePARPtrap™AssayKitisdesignedtomeasurePARP1/DNAcomplexformationinahighthroughputscreeningassayusingfluorescencepolarization(FP).ThePARPtrap™AssayKitcomesinaconvenient96-wellformat,withpurifiedPARP1enzyme,fluorescentlabelednickedDNA,andPARPtrap™assaybufferfor100enzymereactions.ThekeytothePARPtrap™AssayKitisthefluorescentlabelednickedDNA.WithoutthePARreaction,PARP1bindstothefluorescentlabelednickedDNA,resultingintheemissionofhighlypolarizedlight.However,afterautoribosylationofPARP1,thenickedDNAisdissociatedfromPARP1androtatesfreely,emittinglesspolarizedlight(Fig.1).

Figure1.PARPtrap™AssayKitschematic

Synonym(s):
poly(ADP-ribose)polymerase1,poly(ADP-ribosyl)transferase,ADPRT,PARP1
Format:

COMPONENTS:

Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
Upto1yearwhenstoredasrecommended.
Application(s):
GreatforscreeningsmallmoleculesthatenhancePARP1/DNAtrappingfordrugdiscoveryandHTSapplications.
Reference(s):

1.Murai,J.,etal.MolecularCancerTherapeutics2014.13:433-443.
2.Murai,J.,et.al.CancerResearch2012.72:5588-5599.

Notes:

MATERIALSORINSTRUMENTSREQUIREDBUTNOTSUPPLIED:
Fluorescentmicroplatereadercapableofmeasuringfluorescencepolarization
Adjustablemicropipettorandsteriletips
Rotatingorrockerplatform

Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
PARP
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。