Bpsbioscience/Rat PDE7A, GST-tag/60074/10 µg
商品编号:
60074
品牌:
bpsbioscience
市场价:
¥8800.00
美元价:
5280.00
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Species:Rat
HostSpecies:Sf9insectcells
MW:65kDa
Genbank#:NM_031080
Tag(s):N-terminalGST-tag
a.a:147–482(end)
Description:
RatPDE7A(GenBankAccessionNo.NM_031080),a.a.147-482(end),withN-terminalGST-tag,MW=65kDa,expressedinaBaculovirus-infectedSf9cellexpressionsystem.
UniProt
F1LM88
Synonym(s):
PDE7A,phosphodiesterase7A
SpecificActivity:
≥354pmol/min/µg.
UnitDefinition:
Oneunitisdefinedastheamountofenzymethatwillconvert1pmoleof3",5"-cAMPto5"-AMPperminuteat37°C.
AssayConditions:
AssayedusingBPS"sPDE7Akit,Cat.#60370.Assayconditions:10mMTris-HCl,pH7.4,10mMMgCl2,0.05%Tween-20,0.1mg/mlBSA,200µMcAMP,2.5kU5"-nucleotidase,andserialdilutionsofrPDE7Aat37°Cfor20min.Quantifiedby5"-nucleotidasecleavingthe5"-AMPproductandreleasingthephosphategroupwhichisdetectedbyMalachiteGreenReagent.
Formulation:
45mMTris-HCl,pH8.0,124mMNaCl,2.4mMKCl,18mMglutathione,10%glycerol,and3mMDTT.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Han,P.,etal.(1998).GenomicsMar1;48(2):275-6.
2.Yang,G.,etal.(2003).J.Immunol.Dec15;171(12):6414-20.
3.Yamamoto,etal.(2006)Eur.J.Pharmacol.Jul10;541(1-2):106-14.
2.Yang,G.,etal.(2003).J.Immunol.Dec15;171(12):6414-20.
3.Yamamoto,etal.(2006)Eur.J.Pharmacol.Jul10;541(1-2):106-14.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
PDE
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介:为生命科学研究提供ELISA试剂盒(仅供科研)、抗体和抗原(仅供科研)、流式抗体,使您的检测结果更稳定可靠,用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀,吸附性好,空白值低,孔底透明度高,代做ELISA实验等。