Bpsbioscience/Rat PDE2A，他的GST标签/60022/5µg
商品编号：
60022
品牌：
bpsbioscience
市场价：
¥8800.00
美元价：
5280.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Rat
HostSpecies:Sf9insectcells
MW:133kDa
Genbank#:NM_001143847
Tag(s):N-terminalHis-GST-tags
a.a:fulllength
Description:
RatPDE2A(GenBankAccessionNo.NM_00143847),fulllength,withN-terminalHis-GST-tag,MW=133kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem
UniProt
Q01062
Synonym(s):
PDE2A,phosphodiesterase2A
SpecificActivity:
≥2500pmol/min/µg
UnitDefinition:
Oneunitisdefinedastheamountofenzymethatwillconvert1pmoleof3",5"-cAMPto5"-AMPperminuteat37°C.
AssayConditions:
10mMTris-HCl,pH7.4,10mMMgCl2,0.1mg/mlBSA,0.05%Tween-20,200µMcAMP,2.5kU5"-nucleotidase,andserialdilutionsofPDE2Aat37°Cfor20min.Quantifiedby5"-nucleotidasecleavingthe5"-AMPproductandreleasingthephosphategroupwhichisdetectedbyMalachiteGreenReagent.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,4mMglutathione,20%glycerol,and3mMDTT
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Seybold,J.,etal.,Blood105(9),3569-3576(2005).
2.Martinez,S.E.,etal.,Proc.Natl.Acad.Sci.U.S.A.99(20),13260-13265(2002).
ApplicationReference(s):
Ayeast-basedchemicalscreenidentifiesaPDEinhibitorthatelevatessteroidogenesisinmouseLeydigcellsviaPDE8andPDE4inhibition(2013)
Warning(s):
Avoidfreeze/thawcycles.Storingdilutedenzymeisnotrecommended,ifnecessary,usecarrierprotein(BSA0.1-0.5%).
ScientificCategory:
PDE
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
