Bpsbioscience/RAT PDE1B, His-GST-tags/60009/10 µg
商品编号：
60009
品牌：
bpsbioscience
市场价：
¥8800.00
美元价：
5280.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Rat
Host Species: Sf9 insect cells
MW: 89 kDa
Genbank #: NM_022710
Tag(s): N-terminal His-GST-tags
a.a: full length
Description:
Rat PDE1B (GenBank Accession No. NM_022710), full length with N-terminal HIS-GST-tag, MW= 89 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q01066
Synonym(s):
PDE1B, phosphodiesterase 1B
Specific Activity:
≥ 6800 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assay Conditions:
10 mM Tris-HCl,
pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA,
0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and
serial dilutions of PDE1B at 37°C for 20 min.
Quantified by 5'- nucleotidase cleaving the
5' -AMP product and releasing the
phosphate group which is detected by
Malachite Green Reagent.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, 3 mM DTT, 20% glycerol.
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Bender A.T. and Beavo J.A. Proc. Natl. Acad.
Sci. U.S.A. 103 (2), 460-465 (2006).
2. Bender A.T. et al., Proc. Natl. Acad. Sci. U.S.A. 102 (2), 497-502 (2005).
2. Bender A.T. et al., Proc. Natl. Acad. Sci. U.S.A. 102 (2), 497-502 (2005).
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PDE
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
