Bpsbioscience/PDE7B, GST-tag, His-tag/60071/10 µg
商品编号：
60071
品牌：
bpsbioscience
市场价：
¥8800.00
美元价：
5280.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:66kDa
Genbank#:NM_018945
Tag(s):N-terminalGST-tag,C-terminalHis-tag
a.a:109–end
Description:
HumanPDE7BGenBankAccessionNo.NM_018945,(a.a.109-end)withN-terminalGST-tagandC-terminalHis-tag,MW=66kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
UniProt
Q9NP56
Synonym(s):
PDE7B,phosphodiesterase7B
SpecificActivity:
≥226pmol/min/µg.
UnitDefinition:
Oneunitisdefinedastheamountofenzymethatwillconvert1pmoleof3",5"-cAMPto5"-AMPperminuteat37°C.
AssayConditions:
AssayedusingBPS"sPDE7Bkit,Cat.#60371.Assayconditions:10mMTris-HCl,pH7.4,10mMMgCl2,0.05%Tween-20,200µMcAMP,2.5kU5"-nucleotidase,andserialdilutionsofPDE7Bat37°Cfor20min.Quantifiedby5"-nucleotidasecleavingthe5"-AMPproductandreleasingthephosphategroupwhichisdetectedbyMalachiteGreenReagent.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,3mMDTT,20%glycerol,and16mMglutathione
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.Gardner,C.,etal.,Biochem.Biophys.Res.Commun.272(1),186-192(2000)
2.Sasaki,T.,etal.,Biochem.Biophys.Res.Commun.271(3),575-583(2000)
ApplicationReference(s):
1.DiscoveryofaNovelSeriesofPyrazolo[1,5-a]pyrimidine-BasedPhosphodiesterase2AInhibitorsStructurallyDifferentfromN-((1S)-1-(3-Fluoro-4-(trifluoromethoxy)phenyl)-2-methoxyethyl)-7-methoxy-2-oxo-2,3-dihydropyrido[2,3-b]pyrazine-4(1H)-carboxamide(TAK-915),fortheTreatmentofCognitiveDisorders(2017)
2. CharacterizationofthebindingpropertiesofT-773asaPETrADIoligandforphosphodiesterase10A(2015)
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
PDE
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
