Bpsbioscience/PDE6C, GST-tag/60062/5 µg
商品编号：
60062
品牌：
bpsbioscience
市场价：
¥8800.00
美元价：
5280.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:125kDa
Genbank#:NM_006204
Tag(s):N-terminalGST-tag
a.a:fulllength
Description:
HumanPDE6C(GenBankAccessionNo.NM_006204),fulllength,withN-terminalGST-tag,MW=125kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
UniProt
P51160
Synonym(s):
PDE6C,phosphodiesterase6C
SpecificActivity:
≥3,000pmol/min/µg
UnitDefinition:
Oneunitisdefinedastheamountofenzymethatwillconvert1pmoleof3",5"-cGMPto5"-GMPperminuteat37°C.
AssayConditions:
10mMTris-HCl,pH7.4,10mMMgCl2,0.1mg/mlBSA,0.05%Tween-20,200µMcGMP,2.5kU5"-nucleotidase,andserialdilutionsofPDE6Cat37°Cfor60min.Quantifiedby5"-nucleotidasecleavingthe5"-GMPproductandreleasingthephosphategroupwhichisdetectedbyMalachiteGreenReagent.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,16mMGlutathione,20%glycerol,and3mMDTT.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.MartinezS.E.etal.,JBiolChem.2008Sep19;283(38):25913-9.
2.StearnsG.etal.,JNeurosci.2007Dec12;27(50):13866-74.
ApplicationReference(s):
1. DesignandSynthesisofNovelandSelectivePhosphodiesterase2(PDE2a)InhibitorsfortheTreatmentofMemoryDisorders (2017)
2. DiscoveryofSelectivePhosphodiesterase1InhibitorswithMemoryEnhancingProperties (2017)
3. NovelN-hydroxybenzamidesincorporating2-oxoindolinewithunexpectedpotenthistonedeacetylaseinhibitoryeffectsandantitumorcytotoxicity. (2017)
Notes:
ThisproductisamoreactiveversionofformerBPSCat.#60060.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
PDE
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
