Bpsbioscience/PDE4D3, GST-tag/60046/5 µg
商品编号：
60046
品牌：
bpsbioscience
市场价：
¥9100.00
美元价：
5460.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 102.4 kDa
Genbank #: NM_006203
Tag(s): N-terminal GST-tag
a.a: full length
Description:
Human PDE4D3 (GenBank Accession No. NM_006203), full length, with N-terminal GST-tag, MW=102.4 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q08499
Synonym(s):
PDE4D3, phosphodiesterase 4D3
Specific Activity:
≥ 12,900 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assay Conditions:
10 mM Tris-HCl,
pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA,
0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and
serial dilutions of PDE4D3 at 37°C for 20 min.
Quantified by 5'- nucleotidase cleaving the
5' -AMP product and releasing the
phosphate group which is detected by
Malachite Green Reagent.
Formulation:
40 mM Tris-HCl, pH 8.0,
110 mM NaCl, 2.2 mM KCl, 3 mM DTT
and 20% glycerol
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
1. Fidani,L., et al., Eur. J. Neurol. 14 (7), 745-749 (2007).
2. Baillie,G.S., et al., Biochem. J. 404 (1), 71-80 (2007).
2. Baillie,G.S., et al., Biochem. J. 404 (1), 71-80 (2007).
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PDE
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
