Bpsbioscience/PDE4C1, GST-tag/60044/5 µg
商品编号：
60044
品牌：
bpsbioscience
市场价：
¥9100.00
美元价：
5460.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species: Human
Host Species: Sf9 insect cells
MW: 106 kDa
Genbank #: NM_000923
Tag(s): N-terminal GST-tag
a.a: full length
Description:
Human PDE4C1 (GenBank Accession No. NM_000923), full length, with N-terminal GST-tag, MW=106 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
UniProt
Q08493
Synonym(s):
PDE4C, phosphodiesterase 4C
Specific Activity:
≥3,000 pmol/min/µg
Unit Definition:
One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Assay Conditions:
10 mM Tris-HCl,
pH 7.4, 10 mM MgCl2, 1 mM MnCl2,
200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE4C1 at 37°C for 20 min.
Quantified by 5'- nucleotidase cleaving the
5' -AMP product and releasing the
phosphate group which is detected by
Malachite Green Reagent.
Formulation:
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 16 mM glutathione
Format:
Aqueous buffer solution
Storage / Stability:
>6 months at -80°C.
Application(s):
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Reference(s):
Wang H. et al., Biochem J. 2007; 408(2):193-201.
2. Sullivan M., Olsen A.S. and Houslay M.D. Cell. Signal. 1999; 11 (10):735-742.
2. Sullivan M., Olsen A.S. and Houslay M.D. Cell. Signal. 1999; 11 (10):735-742.
Warning(s):
Avoid freeze/thaw cycles.
Scientific Category:
PDE
Product Type:
Recombinant Protein
Data shown is lot-specific. Contact us for specific information on other lots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
