Bpsbioscience/PDE4A10, GST-tag/60038/10 µg
商品编号：
60038
品牌：
bpsbioscience
市场价：
¥9100.00
美元价：
5460.00
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Species:Human
HostSpecies:Sf9insectcells
MW:117kDa
Genbank#:NM_001111309
Tag(s):N-terminalGST-tag
a.a:2–825(end)
Description:
HumanPDE4A10,alsoknownasPDE4Aproteinisoform6,transcriptvariant3(GenBankAccessionNo.NM_001111309),a.a.2-825(end)withN-terminalGST-tag,MW=117kDa,expressedinaBaculovirusinfectedSf9cellexpressionsystem.
UniProt
P27815
Synonym(s):
PDE4Aproteinisoform6,transcriptvariant3,PDE4A10
SpecificActivity:
≥4,400pmol/min/µg
UnitDefinition:
Oneunitisdefinedastheamountofenzymethatwillconvert1pmoleof3",5"-cAMPto5"-AMPperminuteat37°C.
AssayConditions:
AssayedusingBPS"sPDE4AAssayKit,Cat.#60340.Assayconditions:10mMTris-HCl,pH7.4,10mMMgCl2,0.1mg/mlBSA,0.05%Tween-20,200µMcAMP,2.5kU5"-nucleotidase,andserialdilutionsofPDE4A10at37°Cfor20min.Quantifiedby5"-nucleotidasecleavingthe5"-AMPproductandreleasingthephosphategroupwhichisdetectedbyMalachiteGreenReagent.
Formulation:
40mMTris-HCl,pH8.0,110mMNaCl,2.2mMKCl,16mMglutathione,20%glyceroland3mMDTT.
Format:
Aqueousbuffersolution
Storage/StABIlity:
>6monthsat-80°C.
Application(s):
Usefulforthestudyofenzymekinetics,screeninginhibitors,andselectivityprofiling.
Reference(s):
1.McCahill,A.,etal.CellSignal.2008.Nov;20(11):2071-83.
2.McPhee,I.,etal.,CellSignal.2001.Dec;13(12):911-8.
2.McPhee,I.,etal.,CellSignal.2001.Dec;13(12):911-8.
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
PDE
ProductType:
RecombinantProtein
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
