Bpsbioscience/PDE3B Assay Kit/60383/384 reactions
商品编号：
60383
品牌：
bpsbioscience
市场价：
¥14200.00
美元价：
8520.00
产品分类：
其它检测试剂盒
公司分类：
Other_kits
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
Background:
Phosphodiesterases(PDEs)playanimportantroleindynamicregulationofcAMPandcGMPsignaling.PDE3B,alsoknownascGMP-inhibitedphosphodiesterase,isinvolvedinmediatingtheantilipolyticandantiglycogenlyticeffectsofinsulininADIposeandlivertissues.PDE3BcanhydrolyzebothcAMPandcGMP.
Description:
ThePDE3BAssayKitisdesignedforidentificationofPDE3Binhibitorsusingfluorescencepolarization.TheassayisbasedonthebindingofafluorescentnucleotidemonophosphategeneratedbyPDE3Btothebindingagent.ThekeytothePDE3BAssayKitisthespecificbindingagent.Usingthiskit,onlytwosimplestepsonamicrotiterplatearerequiredforPDE3Breactions.First,thefluorescentlylabeledcAMPisincubatedwithasamplecontainingPDE3Bfor1hour.Second,bindingagentisaddedtothereactionmixtoproduceachangeinfluorescentpolarizationthatcanthenbemeasuredusingafluorescencereaderequippedforthemeasurementoffluorescencepolarization.
Synonym(s):
PDE3B,phosphodiesterase3B
SuppliedAs:
ThePDE1Cinhibitorscreeningassaykitcomesinaconvenient96-wellformat,includingpurifiedPDE1Cenzyme,fluorescentlylabeledPDE1Csubstrate(cAMP),bindingagent,andPDEassaybufferfor100enzymereactions.
Format:
COMPONENTS:
Instructionsforuse:
Seeassaykitdatasheetfordetailedprotocol.
Storage/StABIlity:
Atleast6monthsfromdateofreceipt,whenstoredasdirected.Kitcomponentsrequiredifferentstorageconditions.Besuretostoreeachcomponentatthepropertemperatureuponarrival.
Application(s):
GreatforstudyingenzymekineticsandscreeningsmallmolecularinhibitorsfordrugdiscoveryandHTSapplications.
Reference(s):
Chandrasekaran,A.,etal.,CellSignal.2008;20(1):139-53.
Notes:
MATERIALSORINSTRUMENTSREQUIREDBUTNOTSUPPLIED:
Fluorescentmicroplatereadercapabletomeasurefluorescencepolarization
Fluorescentmicroplatereadercapabletomeasurefluorescencepolarization
Warning(s):
Avoidfreeze/thawcycles.
ScientificCategory:
Phosphodiesterase
ProductType:
AssayKit
Datashownislot-specific.Contactusforspecificinformationonotherlots.
品牌介绍
bpsbioscience简介：为生命科学研究提供ELISA试剂盒（仅供科研）、抗体和抗原（仅供科研）、流式抗体，使您的检测结果更稳定可靠，用心服务科研。我公司国内总代理,华东地区代理bpsbioscience 专业经营进口胎牛血清、细胞因子、ELISA试剂盒、细胞、抗体、生物试剂、耗材、培养基、一抗、二抗、其产品吸附均匀，吸附性好，空白值低，孔底透明度高，代做ELISA实验等。
